MED17 Antibody - BSA Free

Western Blot: MED17 Antibody [NBP2-17256] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MED17 antibody [C1C3] (NBP2-17256) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: MED17 Antibody [NBP2-17256] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:200 dilution.

Immunohistochemistry: MED17 Antibody [NBP2-17256] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using MED17(NBP2-17256) antibody at 1:500 dilution.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Bv, Ch, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MED17 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human MED17. The exact sequence is proprietary.
• Predicted Species: Mouse (97%), Rat (98%), Chicken (94%), Rhesus Macaque (100%), Bovine (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MED17
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (85%), Xenopus Laevis (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MED17 Antibody - BSA Free

• Activator-recruited cofactor 77 kDa component
• Cofactor required for Sp1 transcriptional activation subunit 6
• cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa
• CRSP6FLJ10812
• CRSP77cofactor required for Sp1 transcriptional activation, subunit 6 (77kD)
• DRIP77
• DRIP80ARC77
• mediator complex subunit 17Vitamin D3 receptor-interacting protein complex 80 kDa component
• mediator of RNA polymerase II transcription subunit 17
• Thyroid hormone receptor-associated protein complex 80 kDa component
• Transcriptional coactivator CRSP77
• Trap80
• TRAP80CRSP complex subunit 6

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MED17
• Uniprot:
  • Q9NVC6(Human)