MED23 Antibody

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Immunoprecipitation: MED23 Antibody [NB200-337] - Nuclear extract (5 mg) from HeLa cells. Antibody used at 4 ug/5 mg extract.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ChIP, IP, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

MED23 Antibody Summary

Immunogen
A synthetic peptide that maps to a region between residues 1 and 50 of human Cofactor Required for Sp1 transcriptional activation, subunit 3 using the numbering given in Swiss-Prot entry Q9ULK4 (GeneID 9439).
Specificity
NB200-337 is specific for human CRSP 3 protein.
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MED23
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunoprecipitation 1-4 ug/mg lysate
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Western Blot: Not Recommended, use NB 200-339 for WB. *In some cases, the antibody may be diluted further than indicated. Use in chromatin immunoprecipitation reported in scientific literature (PMID 25892301)
Publications
Read Publications using
NB200-337 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25892301).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MED23 Antibody

  • 130 kDa transcriptional co-activator
  • 133 kDa transcriptional co-activator
  • CRSP130
  • DRIP130DKFZp434H0117
  • mediator complex subunit 23CRSP133
  • Protein sur-2 homolog
  • subunit 3, 130kDa
  • Transcriptional coactivator CRSP130
  • Vitamin D3 receptor-interacting protein complex 130 kDa component

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: ChIP, IP, WB (-)

Publications for MED23 Antibody (NB200-337)(5)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ChIP Video Protocol
ChIP Webinar

FAQs for MED23 Antibody (NB200-337) (0)

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Bioinformatics

Gene Symbol MED23
Entrez