MCT2 Antibody

Images

 
Western Blot: MCT2 Antibody [NB300-877] - (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MCT2 Antibody [NB300-877] - Staining of Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MCT2 Antibody Summary

Immunogen
Peptide with sequence C-SNAQSVTSERETNI corresponding to C-Terminus according to NP_004722.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SLC16A7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 40-45 kDa band observed in A549, lung epithelial (lung carcinoma) lysates (calculated MW of 52.2 kDa band according to NP_004722).
Publications
Read Publications using
NB300-877 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MCT2 Antibody

  • MCT 2
  • MCT2solute carrier family 16 (monocarboxylic acid transporters), member 7
  • monocarboxylate transporter 2
  • Solute carrier family 16 member 7
  • solute carrier family 16, member 7 (monocarboxylic acid transporter 2)

Background

Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton-linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family 16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. MCT2 is a high affinity transporter that catalyzes the rapid transport of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate across the plasma membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MCT2 Antibody (NB300-877)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol SLC16A7
Entrez
Uniprot