MCM4 Antibody (S02-8F8)

Western Blot: MCM4 Antibody (S02-8F8) [NBP3-15056] - Western blot detection of MCM4 in Hela, A549, MCF-7, U251 cell lysates using MCM4 Rabbit mAb (1:1000 diluted). Predicted band size: 97kDa. Observed band size: 97kDa.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: S02-8F8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

MCM4 Antibody (S02-8F8) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human MCM4 (Uniprot#: P33991)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MCM4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/2000-1/10000
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for MCM4 Antibody (S02-8F8)

• CDC21 homolog
• CDC21
• CDC54
• DNA replication licensing factor MCM4
• EC 3.6.4.12
• hCdc21
• homolog of S. pombe cell devision cycle 21
• MCM4 minichromosome maintenance deficient 4 (S. cerevisiae)
• MCM4 minichromosome maintenance deficient 4
• MGC33310
• minichromosome maintenance complex component 4
• minichromosome maintenance deficient 4
• P1-Cdc21

Background

MCM4 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MCM4