Mcl-1 Antibody (CL1128) - BSA Free

Western Blot: Mcl-1 Antibody (CL1128) [NBP2-52968] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: MCL1 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of U-2 OS cells using the Anti-MCL1 monoclonal antibody, showing cell cycle dependent staining in mitochondria in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of lymph node in human colon shows strong positivity in the reaction centrum.

Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of human breast cancer shows strong positivity in tumor cells.

Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of human cerebellum shows absence of immunoreactivity (negative control).

Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of human tonsil shows strong immunoreactivity in the reaction centrum.

Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of stomach cancer shows cytoplasmic immunoreactivity in tumor cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL1128
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Mcl-1 Antibody (CL1128) - BSA Free Summary

• Description: Novus Biologicals Mouse Mcl-1 Antibody (CL1128) - BSA Free (NBP2-52968) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from Q07820, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MCL1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
• Control Peptide:

  Mcl-1 Recombinant Protein Antigen (NBP2-52968PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Mcl-1 Antibody (CL1128) - BSA Free

• BCL2L3
• bcl2-L-3
• BCL2L3MGC104264
• Bcl-2-like protein 3
• Bcl-2-related protein EAT/mcl1
• EAT
• induced myeloid leukemia cell differentiation protein Mcl-1
• Mcl1
• Mcl-1
• mcl1/EAT
• MCL1-ES
• MCL1L
• MCL1S
• MGC1839
• myeloid cell leukemia ES
• myeloid cell leukemia sequence 1 (BCL2-related)
• TM

Background

Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Mcl-1 Antibody (NBP2-52968) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Kristen Gumz ELISA Human 04/28/2017

Summary

• Application: ELISA
• Sample Tested: Cancer cell lysates
• Species: Human

Bioinformatics

• Gene Symbol: MCL1