Mcl-1 Antibody (542808) [Unconjugated]

Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line, THP-1 human acute monocytic leukemia cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Mcl-1 Monoclonal Antibody (Catalog # MAB828) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Mcl-1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Mcl-1 knockout A431 cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Mcl-1 Monoclonal Antibody (Catalog # MAB828) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Mcl-1 at approximately 40 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IHC, KO
• Clone: 542808
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Mcl-1 Antibody (542808) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Mcl-1
  Met1-Gly230
  Accession # Q07820
• Specificity: Detects human Mcl-1.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MCL1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Knockout Validated
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mcl-1 Antibody (542808) [Unconjugated]

• BCL2L3
• bcl2-L-3
• BCL2L3MGC104264
• Bcl-2-like protein 3
• Bcl-2-related protein EAT/mcl1
• EAT
• induced myeloid leukemia cell differentiation protein Mcl-1
• Mcl1
• Mcl-1
• mcl1/EAT
• MCL1-ES
• MCL1L
• MCL1S
• MGC1839
• myeloid cell leukemia ES
• myeloid cell leukemia sequence 1 (BCL2-related)
• TM

Background

Mcl-1 (myeloid cell leukemia-1; also known as Bcl-2-like protein 3) is a member of the Bcl-2 family of proteins. Alternative splicing creates two distinct isoforms: 40 kDa Mcl-1L (long; 350 amino acids (aa)) enhances cell survival by inhibiting apoptosis, while 32 kDa Mcl-1S (short; 271 aa with divergence in the last 41 aa) promotes apoptosis. The elimination of Mcl-1L is a required step for DNA damage-induced apoptosis. Mcl-1 can be modified by phosphorylation on S121 and T163 by JNK, which triggers apoptosis, or polyubiquitination, which enhances degradation of Mcl-1. Within the first 230 aa, human Mcl-1 shares ~68% aa identity with mouse and rat Mcl-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mcl-1 Antibody (MAB828)(4)

Publications using MAB828

• Huapeng Zhang, Gongquan Li, Yi Zhang, Jihua Shi, Bing Yan, Hongwei Tang et al. Targeting BET Proteins With a PROTAC Molecule Elicits Potent Anticancer Activity in HCC Cells Frontiers in Oncology 1/14/2020 [PMID: 31993368]
• M Mues, L Karra, D Romero-Moy, A Wandler, MJ Hangauer, O Ksionda, Y Thus, M Lindenberg, K Shannon, MT McManus, JP Roose High-Complexity shRNA Libraries and PI3 Kinase Inhibition in Cancer: High-Fidelity Synthetic Lethality Predictions Cell Rep, 2019-04-09;27(2):631-647.e5. 2019-04-09 [PMID: 30970263] (Flow Cytometry, Human)
• Sathish Kumar Mungamuri, Erica Kay Benson, Shaomeng Wang, Wei Gu, Sam W Lee, Stuart A Aaronson p53-mediated heterochromatin reorganization regulates its cell fate decisions Nature Structural & Molecular Biology 5/1/2012 [PMID: 22466965]
• Sathish Kumar Mungamuri, Erica Kay Benson, Shaomeng Wang, Wei Gu, Sam W Lee, Stuart A Aaronson p53-mediated heterochromatin reorganization regulates its cell fate decisions Nature Structural & Molecular Biology 2012-05-01 [PMID: 22466965] (Western Blot, Human)

Review for Mcl-1 Antibody (MAB828) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Kristen Gumz ELISA Human 04/28/2017

Summary

• Application: ELISA
• Sample Tested: cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: MCL1
• Uniprot:
  • Q07820(Human)