Mcl-1 Antibody (542808) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, IHC, KO
• Clone: 542808
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

Mcl-1 Antibody (542808) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human Mcl-1
  Met1-Gly230
  Accession # Q07820
• Specificity: Detects human Mcl-1.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mcl-1 Antibody (542808) [Alexa Fluor® 405]

• BCL2L3
• bcl2-L-3
• BCL2L3MGC104264
• Bcl-2-like protein 3
• Bcl-2-related protein EAT/mcl1
• EAT
• induced myeloid leukemia cell differentiation protein Mcl-1
• Mcl1
• Mcl-1
• mcl1/EAT
• MCL1-ES
• MCL1L
• MCL1S
• MGC1839
• myeloid cell leukemia ES
• myeloid cell leukemia sequence 1 (BCL2-related)
• TM

Background

Mcl-1 (myeloid cell leukemia-1; also known as Bcl-2-like protein 3) is a member of the Bcl-2 family of proteins. Alternative splicing creates two distinct isoforms: 40 kDa Mcl-1L (long; 350 amino acids (aa)) enhances cell survival by inhibiting apoptosis, while 32 kDa Mcl-1S (short; 271 aa with divergence in the last 41 aa) promotes apoptosis. The elimination of Mcl-1L is a required step for DNA damage-induced apoptosis. Mcl-1 can be modified by phosphorylation on S121 and T163 by JNK, which triggers apoptosis, or polyubiquitination, which enhances degradation of Mcl-1. Within the first 230 aa, human Mcl-1 shares ~68% aa identity with mouse and rat Mcl-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.