MCAM/CD146 Antibody [Alexa Fluor® 488]

• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

MCAM/CD146 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat MCAM/CD146
  Arg19-Lys560
  Accession # Q9EPF2
• Specificity: Detects rat MCAM/CD146 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human MCAM is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

MCAM, also known as CD146 and MUC18, is a type I transmembrane adhesion molecule belonging to the Ig superfamily. It is expressed on all endothelial cells, different epithelial cells, multiple lymphocyte subsets and various tumor cells. Two splice isoforms that differ in their cytoplasmic domains and vasolateral localization have been described. A soluble MCAM is shed and can be detected in circulation. The amino acid sequence of rat MCAM is 72% and 90% identical to that of human and mouse MCAM, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.