MCAM/CD146 Antibody (c264) [FITC]

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Flow Cytometry: MCAM/CD146 Antibody (c264) [FITC] [NBP1-28332] - Thymocytes from a young chicken were isolated and incubated with purified mouse anti-chicken MCAM MAb. The cells were then stained with goat anti-mouse ...read more

Product Details

Summary
Reactivity Ch, TrSpecies Glossary
Applications Flow, IHC, IHC-Fr, IP, ICC
Clone
c264
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

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Catalog# & Conjugate Size Price

MCAM/CD146 Antibody (c264) [FITC] Summary

Immunogen
Mixture of E15 and E16 thymocytes from H.B15, H.B19, and H.B21 chicken embryos
Specificity
Chicken MCAM, Mr 98 kDa (protein core approx. 84 kDa)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MCAM
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Immunocytochemistry

Reactivity Notes

Chicken

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for MCAM/CD146 Antibody (c264) [FITC]

  • CD146 antigen
  • CD146
  • cell surface glycoprotein MUC18
  • Cell surface glycoprotein P1H12
  • L-Gicerin
  • MCAM
  • melanoma adhesion molecule
  • melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen
  • Melanoma-associated antigen A32
  • Melanoma-associated antigen MUC18
  • MUC18
  • MUC18Gicerin

Background

Chicken MCAM, a member of the immunoglobulin superfamily of cell surface receptors, consists of the V-V-C2-C2-C2 Ig domains. It is a single chain glycoprotein with three mRNA splice variants. One has a short cytoplasmic tail, another has a long tail, and the third seems to lack transmembrane and cytoplasmic regions. It is highly homologous to gicerin, a molecule involved in neurite outgrowth and Wilm's kidney tumor progression in the chicken, and to the human melanoma progression molecule MUC18. Chicken MCAM is expressed by c-kit+ hematopoietic progenitor cells in embryonic bone marrow, thymocytes, and capillary endothelial cells. MCAM-expressing cells sorted from bone marrow are enriched in progenitors capable of differentiating into T cells when transferred into the appropriate thymic microenvironment. MCAM has been proposed to be involved in cellular adhesion and homing processes. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-28333

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Bioinformatics

Gene Symbol MCAM
Entrez