MBP Antibody (2H9)

Images

 
Western Blot: MBP Antibody (2H9) [NBP2-22121] - Total human brain lysate was separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was then probed with anti-MBP antibody at 2 ug/ml and ...read more
Immunocytochemistry/ Immunofluorescence: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Immunofluorescence analysis of MSCS cells using Myelin Basic Protein mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: ...read more
Immunohistochemistry-Paraffin: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Immunohistochemical analysis of paraffin-embedded cerebellum tissues using Myelin Basic Protein mouse mAb with DAB staining.
Flow Cytometry: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Flow cytometric analysis of HepG2 cells using Myelin Basic Protein mouse mAb (green) and negative control (red).
Immunohistochemistry-Paraffin: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Immunohistochemical analysis of paraffin-embedded brain tissues using Myelin Basic Protein mouse mAb with DAB staining.
ELISA: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-P
Clone
2H9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MBP Antibody (2H9) Summary

Immunogen
Purified recombinant fragment of human Myelin Basic Protein expressed in E. coli. [UniProt# P02686]
Marker
Oligodendrocyte Marker, Myelin Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MBP
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This Myelin Basic Protein (2H9) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for MBP Antibody (2H9)

  • Hmbpr
  • MBP
  • MGC99675
  • mld
  • Myelin A1 protein
  • myelin basic protein
  • Myelin membrane encephalitogenic protein
  • shi

Background

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MBP Antibody (NBP2-22121). (Showing 1 - 1 of 1 FAQ).

  1. Has this antibody been tested on mouse and rat tissues/cells to show exclusive staining to human cells?
    • For our product NBP2-22121, we have only tested this product in human samples thus far. We have not yet tested for cross reactivity with the mouse or rat proteins. The immunogen for this product shares 70% homology to the mouse sequence and 80% to the rat.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MBP
Entrez
Uniprot