MBP Antibody (2H9) [Janelia Fluor® 646]

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 2H9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 646

MBP Antibody (2H9) [Janelia Fluor® 646] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Purified recombinant fragment of human Myelin Basic Protein expressed in E. coli. [UniProt# P02686]
• Marker: Oligodendrocyte Marker, Myelin Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MBP
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-22121. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Mouse reactivity reported by customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Ammonium sulfate precipitation

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MBP Antibody (2H9) [Janelia Fluor® 646]

• Hmbpr
• MBP
• MGC99675
• mld
• Myelin A1 protein
• myelin basic protein
• Myelin membrane encephalitogenic protein
• shi

Background

Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MBP Antibody (NBP2-22121JF646)(4)

Publications using NBP2-22121JF646

• Lö beta lein L, Linnerbauer M, Zuber F et al. TGF alpha controls checkpoints in CNS resident and infiltrating immune cells to promote resolution of inflammation. Nature Communications 2025-06-19 [PMID: 40537468]
• Mathias Linnerbauer, Lena Lö beta lein, Oliver Vandrey, Anne Peter, Yanan Han, Thanos Tsaktanis, Emile Wogram, Maria Needhamsen, Lara Kular, Lisa Nagel, Julia Zissler, Marie Andert, Lisa Meszaros, Jannis Hanspach, Finnja Zuber, Ulrike J. Naumann, Martin Diebold, Michael A. Wheeler, Tobias Beyer, Lucy Nirschl, Ana Cirac, Frederik B. Laun, Claudia Günther, Jürgen Winkler, Tobias Bäuerle, Maja Jagodic, Bernhard Hemmer, Marco Prinz, Francisco J. Quintana, Veit Rothhammer The astrocyte-produced growth factor HB-EGF limits autoimmune CNS pathology Nature Immunology 2024-02-26 [PMID: 38409259]
• Linnerbauer M, Beyer T, Nirschl L et al. PD-L1 positive astrocytes attenuate inflammatory functions of PD-1 positive microglia in models of autoimmune neuroinflammation Nat Commun 2023-09-09 [PMID: 37689786] (In vivo assay)
• Linnerbauer M, L��lein L, Farrenkopf D et al. Astrocyte-Derived Pleiotrophin Mitigates Late-Stage Autoimmune CNS Inflammation Frontiers in Immunology 2022-01-03 [PMID: 35046956] (In vivo assay)

Bioinformatics

• Gene Symbol: MBP