MBD3 Antibody

Western Blot: MBD3 Antibody [NBP3-18656] - Figure 2A: Western blot was performed on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U937 NE, 60 ug) with antibody against human MBD3 , diluted 1:2,000 in TBS-Tween with 5% skimmed milk. The MW marker (in kDa) is on the left; the location of the protein of interest is indicated on the right. Figure 2B: TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U2OS). Nuclear extracts were prepared and separated by electrophoresis. An antibody directed against the TY1 tag or the antibody against MBD3 (1:2,000 in TBS-Tween containing 5% skimmed milk) were used to detect TTE-MBD3 (lane 1 and 2, respectively). Location of tagged MBD3 is indicated with an arrow.

ELISA: MBD3 Antibody [NBP3-18656] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human MBD3 . The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,890.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MBD3 Antibody Summary

• Description: Novus Biologicals Rabbit MBD3 Antibody (NBP3-18656) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Polyclonal antibody raised in rabbit against human MBD3 (Methyl-CpG-binding domain protein 3), using three different KLH-conjugated synthetic peptides.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MBD3
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:500
  • Western Blot 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antiserum
• Preservative: 0.05% Sodium Azide
• Purity: Whole Antiserum

Alternate Names for MBD3 Antibody

• MBD3
• methyl-CpG binding domain protein 3
• methyl-CpG-binding domain protein 3
• Methyl-CpG-binding protein MBD3

Background

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (4). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (3). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (2). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MBD3
• Uniprot:
  • O95983(Human)