| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3839-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08535 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04590-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00136-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08535 | |||
![Western Blot: MBD3 Antibody (9X1J7) [NBP3-16718] - Western blot analysis of extracts of various cell lines, using MBD3 Rabbit mAb (NBP3-16718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/MBD3-Antibody-9X1J7-Western-Blot-NBP3-16718-img0002.jpg "Western Blot: MBD3 Antibody (9X1J7) [NBP3-16718] - Western blot analysis of extracts of various cell lines, using MBD3 Rabbit mAb (NBP3-16718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: MBD3 Antibody (9X1J7) [NBP3-16718] - Immunohistochemistry of paraffin-embedded human colon carcinoma using MBD3 Rabbit mAb (NBP3-16718) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MBD3-Antibody-9X1J7-Immunohistochemistry-Paraffin-NBP3-16718-img0001.jpg)
![Western Blot: MBD3 Antibody (9X1J7) [NBP3-16718] - Western blot analysis of extracts of various cell lines, using MBD3 Rabbit mAb (NBP3-16718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MBD3-Antibody-9X1J7-Western-Blot-NBP3-16718-img0003.jpg)
![Flow Cytometry: MBD3 Antibody (PCRP-MBD3-1C4) [NBP3-07604] - Flow Cytometric Analysis of PFA-fixed HeLa cells. MBD3 Mouse Monoclonal Antibody (PCRP-MBD3-1C4) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/MBD3-Antibody-PCRP-MBD3-1C4-Flow-Cytometry-NBP3-07604-img0002.jpg "Flow Cytometry: MBD3 Antibody (PCRP-MBD3-1C4) [NBP3-07604] - Flow Cytometric Analysis of PFA-fixed HeLa cells. MBD3 Mouse Monoclonal Antibody (PCRP-MBD3-1C4) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).")
![Protein Array: MBD3 Antibody (PCRP-MBD3-1C4) [NBP3-07604] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MBD3 Mouse Monoclonal Antibody (PCRP-MBD3-1C4).](http://images.novusbio.com/fullsize/MBD3-Antibody-PCRP-MBD3-1C4-Protein-Array-NBP3-07604-img0001.jpg)
![Western Blot: MBD3 Antibody [NBP3-18656] - Figure 2A: Western blot was performed on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U937 NE, 60 ug) with antibody against human MBD3 , diluted 1:2,000 in TBS-Tween with 5% skimmed milk. The MW marker (in kDa) is on the left; the location of the protein of interest is indicated on the right. Figure 2B: TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U2OS). Nuclear extracts were prepared and separated by electrophoresis. An antibody directed against the TY1 tag or the antibody against MBD3 (1:2,000 in TBS-Tween containing 5% skimmed milk) were used to detect TTE-MBD3 (lane 1 and 2, respectively). Location of tagged MBD3 is indicated with an arrow.](http://images.novusbio.com/fullsize/MBD3-Antibody-Western-Blot-NBP3-18656-img0001.jpg "Western Blot: MBD3 Antibody [NBP3-18656] - Figure 2A: Western blot was performed on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U937 NE, 60 ug) with antibody against human MBD3 , diluted 1:2,000 in TBS-Tween with 5% skimmed milk. The MW marker (in kDa) is on the left; the location of the protein of interest is indicated on the right. Figure 2B: TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U2OS). Nuclear extracts were prepared and separated by electrophoresis. An antibody directed against the TY1 tag or the antibody against MBD3 (1:2,000 in TBS-Tween containing 5% skimmed milk) were used to detect TTE-MBD3 (lane 1 and 2, respectively). Location of tagged MBD3 is indicated with an arrow.")
![ELISA: MBD3 Antibody [NBP3-18656] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human MBD3 . The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,890.](http://images.novusbio.com/fullsize/MBD3 Antibody-ELISA-NBP3-18656-img0002.jpg)
![Immunohistochemistry:[NBP3-29255]-Detection of human MBD3 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-MBD3.](http://images.novusbio.com/fullsize/nbp3-29255_rabbit-mbd3-pab-23120251252883.jpg "Immunohistochemistry:[NBP3-29255]-Detection of human MBD3 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-MBD3.")
![Western Blot: MBD3 Antibody [NBP3-04590] - Analysis of extracts of various cell lines, using MBD3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic](http://images.novusbio.com/fullsize/MBD3-Antibody-Western-Blot-NBP3-04590-img0001.jpg "Western Blot: MBD3 Antibody [NBP3-04590] - Analysis of extracts of various cell lines, using MBD3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic")
![Western Blot: MBD3 Antibody [NBP3-00136] - NBP3-00136 at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:37 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/nbp3-00136_rabbit-polyclonal-mbd3-antibody-235202511444423.png "Western Blot: MBD3 Antibody [NBP3-00136] - NBP3-00136 at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:37 kDa (We are unsure as to the identity of these extra bands.)")
![SDS-Page: MBD3 Protein [NBP2-23231]](http://images.novusbio.com/fullsize/MBD3-Protein-SDS-Page-NBP2-23231-img0001.jpg "SDS-Page: MBD3 Protein [NBP2-23231]")