RBBP4/RbAp48 Antibody (13D10)

Images

 
Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Analysis was performed using RbAp48 antibody [13D10]. EasyBlot HRP-conjugated anti mouse IgG antibody was used to detect the primary antibody, and the signal ...read more
Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RbAp48 protein stained by RbAp48 antibody [13D10] diluted at ...read more
Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Detection of RbAp48 in HeLa nuclear extract
Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Immunoprecipitation of RbAp48 protein from HeLa whole cell extract using 5 ug of RbAp48 antibody [13D10]. Western blot analysis was performed using ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP, ChIP
Clone
13D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RBBP4/RbAp48 Antibody (13D10) Summary

Immunogen
The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.
Localization
Nuclear
Specificity
This antibody will recognize human RbAp48.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
RBBP4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunoprecipitation 1:500
  • Western Blot 1:500-1:3000
Application Notes
RBBP4/RbAp48 antibody validated for WB from a verified customer review. Chip reactivity reported in (PMID: 30551478). IHC-Assay dependent.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-41201 in the following applications:

Publications
Read Publications using
NBP1-41201 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34205080).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for RBBP4/RbAp48 Antibody (13D10)

  • CAF-1 p48
  • CAF-1 subunit C
  • CAF-1C
  • CAF-I p48
  • Chromatin assembly factor 1 subunit C
  • Chromatin assembly factor I p48 subunit
  • chromatin assembly factor/CAF-1 p48 subunit
  • histone-binding protein RBBP4
  • MSI1 protein homolog
  • Nucleosome-remodeling factor subunit RBAP48
  • NURF55
  • RBAP48
  • RBBP4
  • RBBP-4
  • retinoblastoma binding protein 4
  • Retinoblastoma-binding protein 4CAF-I 48 kDa subunit
  • Retinoblastoma-binding protein p48

Background

RbAp48-13D10 recognizes full-length RbAp48, a 48a nuclear protein first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). It is a member of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RBBP4/RbAp48 Antibody (NBP1-41201)(9)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 6 applications: B/N, ChIP, Chemotaxis, IF/IHC, In Vivo, WB.


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B/N
(1)
ChIP
(1)
Chemotaxis
(1)
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WB
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(6)
Rat
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Showing Publications 1 - 9 of 9.
Publications using NBP1-41201 Applications Species
Yang B, Zhang W, Sun L et al. Creatine kinase brain-type regulates BCAR1 phosphorylation to facilitate DNA damage repair iScience 2023-05-01 [PMID: 37182100] (ChIP, Human) ChIP Human
Sun JM, Chen HY, Davie JR et al. Differential distribution of unmodified and phosphorylated histone deacetylase 2 in chromatin. J Biol Chem 2007-11-01 [PMID: 17827154] (WB, Human) WB Human
Schultz DC, Friedman JR, Rauscher FJ 3rd. Targeting histone deacetylase complexes via kRAB-zinc finger proteins: the PHD and bromodomains of kAP-1 form a cooperative unit that recruits a novel isoform of the Mi-2alpha subunit of NuRD. Genes Dev 2001-02-01 [PMID: 11230151] (WB, Human) WB Human
Skowyra D, Zeremski M, Neznanov N et al. Differential association of products of alternative transcripts of the candidate tumor suppressor ING1 with the mSin3/HDAC1 transcriptional corepressor complex. J Biol Chem 2001-03-01 [PMID: 11118440] (WB, Human) WB Human
Humphrey GW, Wang Y, Russanova VR et al. Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1. J Biol Chem 2001-03-01 [PMID: 11102443] (WB, Human) WB Human
Khateb A, Deshpande A, Feng Y et al. The ubiquitin ligase RNF5 determines acute myeloid leukemia growth and susceptibility to histone deacetylase inhibitors Nature Communications 2021-09-13 [PMID: 34518534] (B/N, In Vivo) B/N, In Vivo
Mokhtari R, Qorri B, Sambi M et al. 3D Multicellular Stem-Like Human Breast Tumor Spheroids Enhance Tumorigenicity of Orthotopic Xenografts in Athymic Nude Rat Model Cancers 2021-06-03 [PMID: 34205080] (IF/IHC, Rat) IF/IHC Rat
Xiang J, Wang G, Xia T, Chen Z. The depletion of PHF6 decreases the drug sensitivity of T-cell acute lymphoblastic leukemia to prednisolone. Biomedicine & Pharmacotherapy. 1905-07-11 [PMID: 30551478] (Chemotaxis, Human) Chemotaxis Human

Review for RBBP4/RbAp48 Antibody (NBP1-41201) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-41201:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Fugui Li
WB Human 09/23/2014
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Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman

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WB Video Protocol
ICC/IF Video Protocol

FAQs for RBBP4/RbAp48 Antibody (NBP1-41201) (0)

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Recent Reviews

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Fugui Li
09/23/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol RBBP4
Entrez