RBBP4/RbAp48 Antibody (13D10)

Images

 
Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract, B. Control with 2. 5 ug of preimmune mouse IgG, C. Immunoprecipitation ...read more
Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RbAp48 protein stained by RbAp48 antibody [13D10] diluted at ...read more
Chromatin Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Sample: HeLa whole cell lysate/extract A. 5 ug preimmune mouse IgG, B. 5 ug of RbAp48 antibody The precipitated DNA was detected by PCR with ...read more
Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Detection of RbAp48 in HeLa nuclear extract
Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Analysis of HeLa, using RbAp48 antibody at 1:100 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IP
Clone
13D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

RBBP4/RbAp48 Antibody (13D10) Summary

Immunogen
The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.
Localization
Nuclear
Specificity
This antibody will recognize human RbAp48.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
RBBP4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:500
Application Notes
WB: Predicted MW is 47.6 kDa.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-41201 in the following applications:

Publications
Read Publications using
NBP1-41201 in the following applications:

  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for RBBP4/RbAp48 Antibody (13D10)

  • CAF-1 p48
  • CAF-1 subunit C
  • CAF-1C
  • CAF-I p48
  • Chromatin assembly factor 1 subunit C
  • Chromatin assembly factor I p48 subunit
  • chromatin assembly factor/CAF-1 p48 subunit
  • histone-binding protein RBBP4
  • MSI1 protein homolog
  • Nucleosome-remodeling factor subunit RBAP48
  • NURF55
  • RBAP48
  • RBBP4
  • RBBP-4
  • retinoblastoma binding protein 4
  • Retinoblastoma-binding protein 4CAF-I 48 kDa subunit
  • Retinoblastoma-binding protein p48

Background

RbAp48-13D10 recognizes full-length RbAp48, a 48a nuclear protein first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). It is a member of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RBBP4/RbAp48 Antibody (NBP1-41201)(4)

Review for RBBP4/RbAp48 Antibody (NBP1-41201) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-41201:
Filter by Applications
WB
(1)
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(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Fugui Li
WB Human 09/23/2014
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Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman

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Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for RBBP4/RbAp48 Antibody (NBP1-41201) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-41201AF405
Alexa Fluor 488 Labeled NBP1-41201AF488
Alexa Fluor 647 Labeled NBP1-41201AF647
Alexa Fluor 700 Labeled NBP1-41201AF700
DyLight 350 Labeled NBP1-41201UV
DyLight 405 Labeled NBP1-41201V
DyLight 488 Labeled NBP1-41201G
DyLight 680 Labeled NBP1-41201FR
DyLight 755 Labeled NBP1-41201IR

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Bioinformatics Tool for RBBP4/RbAp48 Antibody (NBP1-41201)

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Diseases for RBBP4/RbAp48 Antibody (NBP1-41201)

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PTMs for RBBP4/RbAp48 Antibody (NBP1-41201)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Fugui Li
09/23/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol RBBP4
Entrez