MASTL Antibody

Western Blot: MASTL Antibody [NBP1-83680] - Analysis in control (vector only transfected HEK293T lysate) and mASTL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: MASTL Antibody [NBP1-83680] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: MASTL Antibody [NBP1-83680] - Staining of human rectum shows strong cytoplasmic positivity in lymphoid cells.

Immunohistochemistry-Paraffin: MASTL Antibody [NBP1-83680] - Staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: MASTL Antibody [NBP1-83680] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Immunohistochemistry-Paraffin: MASTL Antibody [NBP1-83680] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MASTL Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: TSQLGFHQSNQWAVDSGGISEEHLGKRSLKRNFELVDSSPCKKIIQNKKTCVEYKHNEMTNCYTNQNTGLTVEVQDLKLSVHKSQQNDCANKEN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MASTL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  MASTL Protein (NBP1-83680PEP)

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24391510)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MASTL Antibody

• FLJ14813EC 2.7.11.1
• Greatwall
• GW
• GWL
• hGWL
• MASTL
• MAST-L
• microtubule associated serine/threonine kinase-like
• Microtubule-associated serine/threonine-protein kinase-like
• RP11-85G18.2
• THC2
• THC2greatwall protein kinase

Background

MASTL (microtubule-associated serine/threonine-protein kinase-like) is an uncharacterized putative serine/threonine kinase which may be involved in megakaryocyte development. Defects in MASTL are the cause of thrombocytopenia type 2 (THC2) a disease characterized by decreased circulating platelets and increased potential for bleeding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MASTL Antibody (NBP1-83680)(1)

Publication using NBP1-83680

• Hegarat N, Vesely C, Vinod PK et al. PP2A/B55 and Fcp1 Regulate Greatwall and Ensa Dephosphorylation during Mitotic Exit. PLoS Genet 2014-01-01 [PMID: 24391510]

Bioinformatics

• Gene Symbol: MASTL