GW182 Antibody

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Biological Strategies: Western Blot: GW182 Antibody [NBP1-28751] - Detection of Human GW182 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified ...read more
Independent Antibodies: Western Blot: GW182 Antibody [NBP1-28751] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GW182 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 575 and 625 of human GW182/glycine-tryptophan protein of 182 kDa (NP_055309.2).
Marker
P/GW Body Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TNRC6A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoblotting
  • Immunoprecipitation 6 ug/mg lysate
Application Notes
Use in Immunoblotting reported in scientific literature (PMID 27557516).
Reviewed Applications
Read 1 Review rated 5
using
NBP1-28751 in the following applications:

Publications
Read Publications using
NBP1-28751 in the following applications:

  • IB
    1 publication
  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GW182 Antibody

  • CAG repeat protein 26
  • CAGH26FLJ22043
  • DKFZp666E117
  • EMSY interactor protein
  • Glycine-tryptophan protein of 182 kDa
  • GW182 autoantigen
  • GW182GW1
  • KIAA1460EDIE
  • MGC75384
  • Protein GW1
  • TNRC6
  • trinucleotide repeat containing 6
  • trinucleotide repeat containing 6A
  • trinucleotide repeat-containing gene 6A protein

Background

GW182 (glycine-tryptophan protein of 182 kDa) is a member of the trinucleotide repeat containing 6 protein family. It functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. GW182 has been found to associate with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibition of GW182 expression delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GW182 Antibody (NBP1-28751)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IB, WB.


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IB
(1)
WB
(5)
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Human
(4)
Mouse
(2)
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Review for GW182 Antibody (NBP1-28751) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-28751:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot GW182 NBP1-28751
Enlarge
reviewed by:
Wei-Ting Lu
WB Human 06/20/2012
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
Lota1

Blocking

Blocking Details5% milk in TBST

Primary Anitbody

Dilution Ratio1/1000

Secondary Antibody

Secondary DescriptionLi-COR Goat anti rabbit antibody IR800
Secondary Concentration1/15000

Details

Detection NotesLi-Cor Odyssey scanner

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GW182 Antibody (NBP1-28751). (Showing 1 - 3 of 3 FAQs).

  1. I have recently tried the NBP1-28751 anti TNRC6A antibody and it works wonders (especially it is long known that it is very hard to get decent antibody against TNRC6 proteins). However there are 3 member of TNRC6 protein, TNRC6A, TNRC6B and TNRC6C which are highly similar. I just wonder if this antibody cross react with other member of TNRC6 protein family? I understand the immunogen information is proprietary, but if you can let me know whether it cross react with TNRC6B and C it will be most helpful!!
    • We tested this antibody via IP with two different antibodies for TNRC6A followed by western blotting with NBP1-28751, confirming the specificity. We have not tested for cross-reactivity with other TNRC6 proteins, however, based on amino acid sequence homology we do not expect NBP1-28751 to cross-react with either TNRC6B or TNRC6C.
  2. What is the % of the gel shown here?
    • For this image we used a 4-12% gradient gel.
  3. Can I use 3-8% gradient?
    • Yes, for this protein that should be fine.

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Recent Reviews

5
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1
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Wei-Ting Lu
06/20/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol TNRC6A
Entrez
Uniprot