Biological Strategies: Western Blot: GW182 Antibody [NBP1-28751] - Detection of Human GW182 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified ...read more
Independent Antibodies: Western Blot: GW182 Antibody [NBP1-28751] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit ...read more
GW182 (glycine-tryptophan protein of 182 kDa) is a member of the trinucleotide repeat containing 6 protein family. It functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. GW182 has been found to associate with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibition of GW182 expression delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for GW182 Antibody (NBP1-28751). (Showing 1 - 3 of 3 FAQs).
I have recently tried the NBP1-28751 anti TNRC6A antibody and it works wonders (especially it is long known that it is very hard to get decent antibody against TNRC6 proteins). However there are 3 member of TNRC6 protein, TNRC6A, TNRC6B and TNRC6C which are highly similar. I just wonder if this antibody cross react with other member of TNRC6 protein family? I understand the immunogen information is proprietary, but if you can let me know whether it cross react with TNRC6B and C it will be most helpful!!
We tested this antibody via IP with two different antibodies for TNRC6A followed by western blotting with NBP1-28751, confirming the specificity. We have not tested for cross-reactivity with other TNRC6 proteins, however, based on amino acid sequence homology we do not expect NBP1-28751 to cross-react with either TNRC6B or TNRC6C.
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