MASTL Antibody - BSA Free

Western Blot: MASTL Antibody [NBP1-22987] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MASTL antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: MASTL Antibody [NBP1-22987] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MASTL used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Immunoprecipitation: MASTL Antibody [NBP1-22987] - Detection of human MASTL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MASTL antibody NBP1-22987 (lot NBP1-22987-4) used for IP at 3 ug per reaction. MASTL was also immunoprecipitated by a previous lot of this antibody (lot NBP1-22987-3). For blotting immunoprecipitated MASTL, NBP1-22987 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MASTL Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 550 and 600 of human microtubule associated serine/threonine kinase-like using the numbering given in entry NP_116233.2 (GeneID 84930).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MASTL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MASTL Antibody - BSA Free

• FLJ14813EC 2.7.11.1
• Greatwall
• GW
• GWL
• hGWL
• MASTL
• MAST-L
• microtubule associated serine/threonine kinase-like
• Microtubule-associated serine/threonine-protein kinase-like
• RP11-85G18.2
• THC2
• THC2greatwall protein kinase

Background

MASTL (microtubule-associated serine/threonine-protein kinase-like) is an uncharacterized putative serine/threonine kinase which may be involved in megakaryocyte development. Defects in MASTL are the cause of thrombocytopenia type 2 (THC2) a disease characterized by decreased circulating platelets and increased potential for bleeding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MASTL Antibody (NBP1-22987)(1)

Publication using NBP1-22987

• Della Monica R, Visconti R, Cervone N et al. Fcp1 phosphatase controls Greatwall kinase to promote PP2A-B55 activation and mitotic progression. Elife. 2015-12-14 [PMID: 26653855]

Bioinformatics

• Gene Symbol: MASTL
• Entrez:
  • 84930(Human)
• Uniprot:
  • Q96GX5()