MafA Antibody

Western Blot: MafA Antibody [NBP2-24636] - Analysis of MafA in human liver lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver lysate and 4) rat liver lysate using this antibody. 25 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Hu, Mu, Rt, Ma-Op
• Applications: WB, ChIP, ICC/IF, IHC, ChIP, ICC/IF (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MafA Antibody Summary

• Immunogen: A portion of amino acids 300-350 of human MafA was used as the immunogen.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAFA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation reported in scientific literature (PMID 24509877)
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 24509877)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 24509877)
  • Western Blot 0.25-0.5ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for MafA Antibody

• hMafA
• Pancreatic beta-cell-specific transcriptional activator
• RIPE3b1
• transcription factor MafA
• Transcription factor RIPE3b1
• v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian)
• V-maf musculoaponeurotic fibrosarcoma oncogene homolog A

Background

Insulin gene expression is regulated by several islet-enriched transcription factors. However, MafA is the only beta cell-specific activator. MafA selectively induces endogenous insulin transcription in non-beta cells. MafA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MafA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MafA Antibody (NBP2-24636)(1)

Publication using NBP2-24636

• Brundage ME, Tandon P, Eaves DW et al. MAF mediates crosstalk between Ras-MAPK and mTOR signaling in NF1. Oncogene. 2014-02-10 [PMID: 24509877] (WB, IHC-P, ICC/IF, Chemotaxis, Human, Mouse)
  
  Details:
  Malignant peripheral nerve sheath (MPNST) mouse tumor cell lines, normal mouse Schwann cells, inducible MAF (iMAF)-transduced MPNST cells, & normal human Schwann cells from trauma victims, Xenograft mouse iMAF tumor models. WB: Figs 2b, 5f, 6d, 8a, IHC (P

Bioinformatics

• Gene Symbol: MAFA
• Entrez:
  • 395881(Chicken)
  • 389692(Human)
  • 378435(Mouse)
  • 366949(Rat)
• Uniprot:
  • Q8NHW3(Human)