MafA Antibody

Immunocytochemistry/Immunofluorescence: MafA Antibody [NBP1-00121] - Analysis of MafA in 8-week old mouse pancretic cryosection using anti-MafA antibody (red). Image from verified customer review.

Immunohistochemistry-Paraffin: MafA Antibody [NBP1-00121] - Section of mouse pancreatic islet. Antibody: Affinity purified rabbit anti-MafA (Cat. No. NBP1-00121) used at a dilution of 1:250.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.05 mg/ml

MafA Antibody Summary

• Description: Novus Biologicals Rabbit MafA Antibody (NBP1-00121) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-MafA Antibody: Cited in 18 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 300 and the C-terminus (residue 359) of mouse v-maf musculoaponeurotic fibrosarcoma oncogene homolog A using the numbering given in entry NP_919331.1 (GeneID 378435).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAFA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID: 28705881)/ verified customer review
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Frozen Reported in scientific literature (PMID: 31201281)
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot Reported in scientific literature (PMID: 26495868)
• Application Notes: Epitope exposure with citrate bufferexposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26495868).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.05 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MafA Antibody

• hMafA
• Pancreatic beta-cell-specific transcriptional activator
• RIPE3b1
• transcription factor MafA
• Transcription factor RIPE3b1
• v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian)
• V-maf musculoaponeurotic fibrosarcoma oncogene homolog A

Background

MafA is a member of the Maf basic leucine-zipper containing transcription factor family. MafA is an islet-enriched protein that plays a role in beta cell-specific and glucose-responsive insulin gene transcription. MafA is expressed exclusively in cell populations that will become islet beta cells. Alternate names for MafA include v-maf musculoaponeurotic fibrosarcoma oncogene homolog A, transcription factor MafA, pancreatic beta-cell-specific transcriptional activator, hMafA, and RIPE3b1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MafA Antibody (NBP1-00121)(18)

Publications using NBP1-00121

• Liu Y, Kepple JD, Shalev A, S Hunter C LDB1-mediated transcriptional complexes are sensitive to islet stress Islets 2022-12-31 [PMID: 34968409] (WB, Rat)
• Walker EM, Cha J, Tong X Et al. Sex-biased islet beta cell dysfunction is caused by the MODY MAFA S64F variant by inducing premature aging and senescence in males Cell reports 2021-10-12 [PMID: 34644565]
• Tong X, Stein R. Lipid Droplets Protect Human ?-Cells From Lipotoxicity-Induced Stress and Cell Identity Changes Diabetes 2021-11-01 [PMID: 34433630]
• Yang, X, Graff, S M Et al. Coregulator Sin3a Promotes Postnatal Murine beta -Cell Fitness by Regulating Genes in Ca2+ Homeostasis, Cell Survival, Vesicle Biosynthesis, Glucose Metabolism, and Stress Response. Diabetes 2020-06-01 [PMID: 32245798] (ICC/IF, Human)
• Shrestha N, Liu T, Ji Y et Al. Sel1L-Hrd1 ER-associated degradation maintains beta-cell identity via TGF- beta signaling J. Clin. Invest. 2020-03-17 [PMID: 32182217] (ICC/IF, Mouse)
• Jaafar R, Tran S, Shah A et al. mTORC1 to AMPK switching underlies beta-cell metabolic plasticity during maturation and diabetes J. Clin. Invest. 2019-07-02 [PMID: 31265435] (ICC/IF, Mouse, Human)
• Spaeth JM, Liu JH, Peters D et al. The Pdx1 Bound Swi/Snf Chromatin Remodeling Complex Regulates Pancreatic Progenitor Cell Proliferation and Mature Islet beta Cell Function Diabetes 2019-06-14 [PMID: 31201281] (IF/IHC, IHC-Fr, Mouse)
• Wade AK, Liu Y, Bethea MM et al. LIM-domain transcription complexes interact with ring-finger ubiquitin ligases and thereby impact islet beta-cell function J. Biol. Chem. 2019-06-11 [PMID: 31186351] (WB, Mouse)
• Wilson, CS;Spaeth, JM;Karp, J;Stocks, BT;Hoopes, EM;Stein, RW;Moore, DJ; B lymphocytes protect islet beta cells in diabetes prone NOD mice treated with imatinib JCI Insight 2019-04-09 [PMID: 30964447] (IF/IHC, Mouse)
• Huang C, Walker EM, Dadi PK, Hu R et Al. Synaptotagmin 4 Regulates Pancreatic ? Cell Maturation by Modulating the Ca(2+) Sensitivity of Insulin Secretion Vesicles Dev Cell 2018-04-17 [PMID: 29656931]
• Spaeth JM, Gupte M, Perelis M et al. Defining a Novel Role for the PDX1 Transcription Factor in Islet B Cell Maturation and Proliferation During Weaning Diabetes 2017-07-13 [PMID: 28705881] (ICC/IF, Mouse)
• Conrad E, Dai C, Spaeth J et al. The MAFB transcription factor impacts islet a-cell function in rodents and represents a unique signature of primate islet b-cells. Am. J. Physiol. Endocrinol. Metab. 2016-01-01 [PMID: 26554594] (IF/IHC, Human)
• Galloway J R, Bethea M et al. SSBP3 Interacts With Islet-1 and Ldb1 to Impact Pancreatic beta-Cell Target Genes. Mol Endocrinol 2015-01-12 [PMID: 26495868] (WB, Mouse)
• Scoville DW, Cyphert HA, Liao L et al. MLL3 and MLL4 methyltransferases bind to the MAFA and MAFB transcription factors to regulate islet b-cell function Diabetes. 2015-07-15 [PMID: 26180087]
• Okano S, Hayasaka K, Igarashi M et al. Characterization of age-associated alterations of islet function and structure in diabetic mutant cryptochrome 1 transgenic mice. J Diabetes Investig 2013-09-13 [PMID: 24843691] (IF/IHC, Mouse)
• DeFalco T, Takahashi S, Capel B et al. Two distinct origins for Leydig cell progenitors in the fetal testis. Dev Biol 2011-04-01 [PMID: 21255566]
• Noso S, Kataoka K, Kawabata Y et al. Insulin transactivator MafA regulates intrathymic expression of insulin and affects susceptibility to type 1 diabetes. Diabetes 2010-10-01 [PMID: 20682694]
• Raum JC, Hunter CS, Artner I et al. Islet beta-cell-specific MafA transcription requires the 5'-flanking conserved region 3 control domain. Mol Cell Biol 2010-09-01 [PMID: 20584984]

Review for MafA Antibody (NBP1-00121) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IF Mouse 09/11/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse Pancreas
• Species: Mouse
• Lot: 1

Bioinformatics

• Gene Symbol: MAFA
• Entrez:
  • 378435(Mouse)
• Uniprot:
  • NP_919331.1(Mouse)