MafA Antibody (BLR067G) - BSA Free

Western Blot: MafA Antibody (BLR067G) [NBP2-78707] - Detection of human and mouse MafA by WB of HEK293T transfected with myc tagged human or mouse MafA, MafB or c-Maf using this antibody, rabbit anti-MafB recombinant monoclonal [BLR046F], and rabbit anti-c-Maf recombinant monoclonal [BLR045F]. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332 ). Lower panel: Rabbit anti-Myc.

Immunocytochemistry/Immunofluorescence: MafA Antibody (BLR067G) [NBP2-78707] - Rabbit anti-MafA recombinant monoclonal antibody [BLR067G]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry-Paraffin: MafA Antibody (BLR067G) [NBP2-78707] - Rabbit anti-MafA recombinant monoclonal antibody [BLR067G] Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Western Blot: MafA Antibody (BLR067G) [NBP2-78707] - Detection of mouse MafA by WB of Beta-TC-6, AlphaTC1 Clone 9, and NIH 3T3 lysate using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332 ).

Immunohistochemistry-Paraffin: MafA Antibody (BLR067G) [NBP2-78707] - Detection of human MafA (red) in FFPE pancreatic carcinoma by IHC-IF using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: Opal™. Counterstain: DAPI (blue).

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clone: BLR067G
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MafA Antibody (BLR067G) - BSA Free Summary

• Description: Novus Biologicals Rabbit MafA Antibody (BLR067G) - BSA Free (NBP2-78707) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Immunogen was a peptide representing a region between residue 125 and 175 of human MAF bZIP transcription factor A using the numbering given in entry NP_963883.2 (Gene ID 389692).
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAFA
• Purity: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Application Notes: Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. IHC and ICC/IF: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Zinc-fixative (JB fix) is recommended to enhance staining.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2.
• Preservative: 0.09% Sodium Azide
• Purity: Protein A or G purified from cell culture supernatant

Notes

This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for MafA Antibody (BLR067G) - BSA Free

• hMafA
• Pancreatic beta-cell-specific transcriptional activator
• RIPE3b1
• transcription factor MafA
• Transcription factor RIPE3b1
• v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian)
• V-maf musculoaponeurotic fibrosarcoma oncogene homolog A

Background

Insulin gene expression is regulated by several islet-enriched transcription factors. However, MafA is the only beta cell-specific activator. MafA selectively induces endogenous insulin transcription in non-beta cells. MafA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MafA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAFA
• Entrez:
  • NP_963883.2(Human)