M-CSFR/CD115 Antibody (61708) [Phycoerythrin]

Human peripheral blood monocytes were stained with Mouse Anti-Human M-CSF R PE-conjugated Monoclonal Antibody (Catalog # FAB329P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 61708
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

M-CSFR/CD115 Antibody (61708) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human M-CSF R
  Ile20-Glu512 (Pro54Ala)
  Accession # P07333.2
• Specificity: Detects human M-CSF R in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) GM-CSF R alpha or rhGM‑CSF R beta .
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSF1R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for M-CSFR/CD115 Antibody (61708) [Phycoerythrin]

• CD115 antigen
• CD115
• c-fms
• colony stimulating factor 1 receptor
• CSF1R
• CSF-1-R
• CSFR
• EC 2.7.10.1
• FMS proto-oncogene
• FMSFIM2
• macrophage colony stimulating factor I receptor
• macrophage colony-stimulating factor 1 receptor
• McDonough feline sarcoma viral (v-fms) oncogene homolog
• M-CSF R
• MCSFR
• M-CSFR
• Proto-oncogene c-Fms

Background

M-CSF Receptor (M-CSF R), the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF Receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Human M-CSF Receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain, and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75%, and 76% aa identity with mouse, rat, bovine, canine, feline, and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF Receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK, and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC (3, 4). M-CSF R and Integrin  alpha _v beta ₃ share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

1. deParseval, N. et al. (1993) Nucleic Acids Res. 21:750.
2. Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311.
3. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
4. Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88.
5. Rovida, E. et al. (2001) J. Immunol. 166:1583.
6. Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128.
7. Dai, X. et al. (2002) Blood 99:111.
8. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
9. Li, M. et al. (2004) J. Neurochem. 91:623.
10. Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for M-CSFR/CD115 Antibody (FAB329P)(5)

Publications using FAB329P

• Bigley V, McGovern N, Milne P, Dickinson R, Pagan S, Cookson S, Haniffa M, Collin M Langerin-expressing dendritic cells in human tissues are related to CD1c+ dendritic cells and distinct from Langerhans cells and CD141high XCR1+ dendritic cells. J Leukoc Biol, 2014-12-16;97(4):627-34. 2014-12-16 [PMID: 25516751] (Flow Cytometry, Human)
• Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R. PLoS ONE, 2014-01-29;9(1):e84417. 2014-01-29 [PMID: 24489649] (Flow Cytometry, Human)
• Kyung-Dal Choi, Maxim Vodyanik, Igor I. Slukvin The Hematopoietic Differentiation and Production of Mature Myeloid Cells from Human Pluripotent Stem Cells Nature Protocols 3/5/2011 [PMID: 21372811]
• Ghirelli C, Zollinger R, Soumelis V Systematic cytokine receptor profiling reveals GM-CSF as a novel TLR-independent activator of human plasmacytoid predendritic cells. Blood, 2010-04-09;115(24):5037-40. 2010-04-09 [PMID: 20382843] (Flow Cytometry, Human)
• Ji JD, Park-Min KH, Shen Z, Fajardo RJ, Goldring SR, McHugh KP, Ivashkiv LB Inhibition of RANK expression and osteoclastogenesis by TLRs and IFN-gamma in human osteoclast precursors. J. Immunol., 2009-11-04;183(11):7223-33. 2009-11-04 [PMID: 19890054] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CSF1R
• Uniprot:
  • P07333.2()