M-CSFR/CD115 Antibody (12-3A3-1B10) - BSA Free

Flow Cytometry: M-CSF R/CD115 Antibody (12-3A3-1B10) [NBP1-43362] - Analysis using the Biotin conjugate of NBP1-43362. Staining of normal human peripheral blood cells with 0.06 ug of Rat IgG2b K Isotype Control Biotin (open histogram) or 0.06 ug of Anti-Human CD115 (c-fms) Biotin (filled histogram) followed by Streptavidin PE.

• Reactivity: Hu
• Applications: WB, Flow, IHC, IP
• Clone: 12-3A3-1B10
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

M-CSFR/CD115 Antibody (12-3A3-1B10) - BSA Free Summary

• Additional Information: Clone 12-3A3-1B10 was used by HLDA to establish CD designation.
• Immunogen: The immunogen for this antibody was CD115.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: CSF1R
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for M-CSFR/CD115 Antibody (12-3A3-1B10) - BSA Free

• CD115 antigen
• CD115
• c-fms
• colony stimulating factor 1 receptor
• CSF1R
• CSF-1-R
• CSFR
• EC 2.7.10.1
• FMS proto-oncogene
• FMSFIM2
• macrophage colony stimulating factor I receptor
• macrophage colony-stimulating factor 1 receptor
• McDonough feline sarcoma viral (v-fms) oncogene homolog
• M-CSF R
• MCSFR
• M-CSFR
• Proto-oncogene c-Fms

Background

CD115 or Colony-Stimulating Factor-1 Receptor (CSF-1R) is expressed on cells committed to the monocyte lineage and osteoclasts. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subsequently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. CSF-1R activation promotes the survival, proliferation and differentiation of mononuclear phagocytes and the spreading and motility of macrophages. In osteoclasts, CSF-1R synergizes with RANKL to regulate the differentiation of mononuclear phagocytes to osteoclasts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for M-CSFR/CD115 Antibody (NBP1-43362). (Showing 1 - 1 of 1 FAQ).

1. A short time ago, I had bought MCSF receptor (NBP1-43362) from your company. Seeing the datasheet says Don't freeze, I'm confused because I don't know if I can store the diluted antibody at -20C.
   • NBP1-43362 should be stored at 4C, and should not be frozen. We do not recommend freezing the antibody as it may adversely affect the antibody's function.

Bioinformatics

• Gene Symbol: CSF1R