M-Cadherin/Cadherin-15 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: M-Cadherin/Cadherin-15 Antibody [NBP1-88101] - Staining of human cell line RH-30 shows localization to cytosol & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: M-Cadherin/Cadherin-15 Antibody [NBP1-88101] - Staining of human cerebellum shows weak cytoplasmic positivity in cells in granular layer.
Orthogonal Strategies: Immunohistochemistry-Paraffin: M-Cadherin/Cadherin-15 Antibody [NBP1-88101] - Analysis in human skeletal muscle and skin tissues. Corresponding CDH15 RNA-seq data are presented for the same ...read more
Immunohistochemistry-Paraffin: M-Cadherin/Cadherin-15 Antibody [NBP1-88101] - Staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: M-Cadherin/Cadherin-15 Antibody [NBP1-88101] - Staining of human skin shows no positivity in squamous epithelial cells as expected.
Immunohistochemistry-Paraffin: M-Cadherin/Cadherin-15 Antibody [NBP1-88101] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: M-Cadherin/Cadherin-15 Antibody [NBP1-88101] - Staining of human prostate shows moderate to strong cytoplasmic positivity in smooth muscle cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

M-Cadherin/Cadherin-15 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TFSARDPDTEQLQRLSYSKDYDPEDWLQVDAATGRIQTQHVLSPASPFLKGGWYRAIVLAQDDASQPRTATGTLSIEILEVNDHAPVLAPPPPGSLCSEPHQGPGLLLGATDEDLPPHGAPFHFQLSPRLPELGRNWSLSQVNVSH
Specificity
Specificity of human M-Cadherin/Cadherin-15 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDH15
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (87%), Rat (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for M-Cadherin/Cadherin-15 Antibody

  • cadherin 15, M-cadherin (myotubule)
  • cadherin 15, type 1, M-cadherin (myotubule)
  • Cadherin-14
  • Cadherin-15
  • cadherin-3
  • CDH14
  • CDH14cadherin-14
  • CDH15
  • CDH3cadherin-15
  • CDHM
  • MCAD
  • MCadherin
  • M-Cadherin
  • MRD3
  • Muscle cadherin
  • muscle-cadherin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CDH15
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