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ACADVL Antibody

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Western Blot: ACADVL Antibody [NBP2-15238] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: ACADVL Antibody [NBP2-15238] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: ACADVL Antibody [NBP2-15238] - ACADVL antibody detects ACADVL protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. ...read more
Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238] - Rat liver. ACADVL stained by ACADVL antibody [N1C1] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: ACADVL Antibody [NBP2-15238] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE, antibody diluted at 1:3000.
Western Blot: ACADVL Antibody [NBP2-15238] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG ...read more
Western Blot: ACADVL Antibody [NBP2-15238] - Wild-type (WT) and ACADVL knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody diluted at 1:1000. ...read more
Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238] - H358 xenograft, using ACADVL antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238] - Human A549 xenograft. ACADVL antibody [N1C1] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238] - Mouse kidney. ACADVL stained by ACADVL antibody [N1C1] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ACADVL Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ACADVL. The exact sequence is proprietary.
Localization
Mitochondrion inner membrane
Predicted Species
Bovine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACADVL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-15238.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 1% BSA, 20% Glycerol (pH7).
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for ACADVL Antibody

  • ACAD6
  • acyl-CoA dehydrogenase, very long chain
  • acyl-Coenzyme A dehydrogenase, very long chain
  • EC 1.3.99
  • EC 1.3.99.-
  • LCACD
  • very long-chain specific acyl-CoA dehydrogenase, mitochondrial
  • VLCAD

Background

The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: ACADM Antibody (JE48-09) [NBP2-80447] - Analysis of ACADM on mouse liver tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence: ACADM Antibody (JE48-09) [NBP2-80447] - ICC staining of ACADM in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1:50) for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).
Immunocytochemistry/Immunofluorescence: ACADM Antibody (JE48-09) [NBP2-80447] - ICC staining of ACADM in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1:100) for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

M-Cadherin/Cadherin-15 ...
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M‑Cadherin/Cadherin-15 was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Human M‑Cadherin/Cadherin-15 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4096) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter­stained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
C2C12 mouse myoblast cell line was stained with Sheep Anti-Human M‑Cadherin/Cadherin‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4096, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human M-Cadherin/Cadherin-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4096) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for M-Cadherin/Cadherin-15 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Immunocytochemistry/Immunofluorescence: CPT2 Antibody (SN06-70) [NBP2-67699] - Staining CPT2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CPT2 Antibody (SN06-70) [NBP2-67699] - Analysis of paraffin-embedded human kidney tissue using anti-CPT2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CPT2 Antibody (SN06-70) [NBP2-67699] - Analysis of paraffin-embedded human liver cancer tissue using anti-CPT2 antibody. Counter stained with hematoxylin.
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Applications: ICC/IF, IHC, IHC-P, WB
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Immunocytochemistry/Immunofluorescence: ACADS Antibody [NBP1-89290] - Staining of human cell line Hep G2 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ACADS Antibody [NBP1-89290] - Staining of human tonsil shows granular cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: ACADS Antibody [NBP1-89290] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
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Immunohistochemistry-Paraffin: ACADL Antibody [NBP1-89289] - Analysis in human thyroid gland and cerebral cortex tissues. Corresponding ACADL RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ACADL Antibody [NBP1-89289] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ACADL Antibody [NBP1-89289] - Staining of human lung shows moderate to strong granular cytoplasmic positivity in macrophages.
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Western Blot: Caldesmon/CALD1 Antibody [NBP1-85702] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85702] - Staining in human smooth muscle and skeletal muscle tissues using anti-CALD1 antibody. Corresponding CALD1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/Immunofluorescence: Caldesmon/CALD1 Antibody [NBP1-85702] - Staining of human cell line U-2 OS shows localization to plasma membrane and actin filaments. Antibody staining is shown in green.
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Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Jurkat cells with PPAR alpha/NR1C1 Antibody (3B6/PPAR) NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on U-87 cells with PPAR alpha/NR1C1 Antibody [3B6/PPAR] NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
(1 Review)
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Immunocytochemistry/Immunofluorescence: HADHA Antibody [NBP1-83387] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HADHA Antibody [NBP1-83387] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: HADHA Antibody [NBP1-83387] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
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Western Blot: MCT1/SLC16A1 Antibody [NBP1-59656] - Monocarboxylic acid transporter 1 Antibody [NBP1-59656] - Jurkat cell lysate, concentration 2.5 ug/mL.
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Western Blot: MCAT Antibody (2F2) [H00027349-M01] - MT monoclonal antibody (M01), clone 2F2 Analysis of MT expression in HeLa.
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Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.
Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.
Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.
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Western Blot: Metallothionein-1B Overexpression Lysate (Adult Normal) [NBP2-10437] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Metallothionein-1B.
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Western Blot: MTTP Antibody [NBP1-62489] - Adult mouse small intestine homogenate, 20 ug total protein per lane. Antibody at 1:1000. Secondary HRP conjugated antibody at 1:5000. Bands visualized with Western Chemiluminescence HRP substrate, imaged with Syngene imaging system. WB image submitted by a verified customer review.
Western Blot: MTTP Antibody [NBP1-62489] - HepG2 cell lysate.
(1 Review)
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ACADVL Antibody
NBP2-15238
ACADVL Antibody
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-P
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol ACADVL
Uniprot