ACADVL Antibody

Images

 
Western Blot: ACADVL Antibody [NBP2-15238] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: ACADVL Antibody [NBP2-15238] - ACADVL antibody detects ACADVL protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. ...read more
Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238] - Rat liver. ACADVL stained by ACADVL antibody [N1C1] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: ACADVL Antibody [NBP2-15238] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE, antibody diluted at 1:3000.
Western Blot: ACADVL Antibody [NBP2-15238] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG ...read more
Western Blot: ACADVL Antibody [NBP2-15238] - Wild-type (WT) and ACADVL knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody diluted at 1:1000. ...read more
Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238] - H358 xenograft, using ACADVL antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238] - Human A549 xenograft. ACADVL antibody [N1C1] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238] - Mouse kidney. ACADVL stained by ACADVL antibody [N1C1] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ACADVL Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ACADVL. The exact sequence is proprietary.
Localization
Mitochondrion inner membrane
Predicted Species
Bovine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACADVL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-15238.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 1% BSA, 20% Glycerol (pH7).
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for ACADVL Antibody

  • ACAD6
  • acyl-CoA dehydrogenase, very long chain
  • acyl-Coenzyme A dehydrogenase, very long chain
  • EC 1.3.99
  • EC 1.3.99.-
  • LCACD
  • very long-chain specific acyl-CoA dehydrogenase, mitochondrial
  • VLCAD

Background

The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ACADVL Antibody (NBP2-15238)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACADVL
Uniprot