ACADVL Antibody

Western Blot: ACADVL Antibody [NBP2-15238] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: ACADVL Antibody [NBP2-15238] - ACADVL antibody detects ACADVL protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ACADVL stained by ACADVL antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.

Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238] - Rat liver. ACADVL stained by ACADVL antibody [N1C1] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: ACADVL Antibody [NBP2-15238] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE, antibody diluted at 1:3000.

Western Blot: ACADVL Antibody [NBP2-15238] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: ACADVL Antibody [NBP2-15238] - Wild-type (WT) and ACADVL knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238] - H358 xenograft, using ACADVL antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238] - Human A549 xenograft. ACADVL antibody [N1C1] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238] - Mouse kidney. ACADVL stained by ACADVL antibody [N1C1] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ACADVL Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ACADVL. The exact sequence is proprietary.
• Localization: Mitochondrion inner membrane
• Predicted Species: Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACADVL
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for ACADVL Antibody

• ACAD6
• acyl-CoA dehydrogenase, very long chain
• acyl-Coenzyme A dehydrogenase, very long chain
• EC 1.3.99
• EC 1.3.99.-
• LCACD
• very long-chain specific acyl-CoA dehydrogenase, mitochondrial
• VLCAD

Background

The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ACADVL Antibody (NBP2-15238)(2)

Publications using NBP2-15238

• Marumo T, Maduka CV, Ural E et al. Flavinated SDHA underlies the change in intrinsic optical properties of oral cancers Communications biology 2023-11-09 [PMID: 37945749] (ICC/IF, WB, Human)
  
  Details:
  1:500 dilution WB
• Tcheng M, Roma A, Ahmed N et al. Very long chain fatty acid metabolism is required in acute myeloid leukemia Blood 2021-03-15 [PMID: 33720355]
  
  Details:
  Antibody used in co-immunoprecipitation

Bioinformatics

• Gene Symbol: ACADVL
• Uniprot:
  • P49748(Human)