HADHA Antibody

Western Blot: HADHA Antibody [NBP1-83387] - Analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-HADHA antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: HADHA Antibody [NBP1-83387] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: HADHA Antibody [NBP1-83387] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.

Western Blot: HADHA Antibody [NBP1-83387] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: HADHA Antibody [NBP1-83387] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: HADHA Antibody [NBP1-83387] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: HADHA Antibody [NBP1-83387] - Staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

HADHA Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: IEYLEEVAITFAKGLADKKISPKRDKGLVEKLTAYAMTIPFVRQQVYKKVEEKVRKQTKGLYPAPLKIIDVVKTGIEQGSDAGYLCESQKFGELVMTKESKALMGLYHGQVLCKKNKFGAPQKDVKHLAILGAGLMGAGIAQVSVDK
• Specificity: Specificity of human, mouse HADHA antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HADHA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control:

  HADHA Knockout 293T Cell Lysate

• Control Peptide:

  HADHA Protein (NBP1-83387PEP)

Reactivity Notes

Rat (85%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for HADHA Antibody

• 3-ketoacyl-Coenzyme A (CoA) thiolase, alpha subunit
• 3-oxoacyl-CoA thiolase
• 78 kDa gastrin-binding protein
• ECHA
• gastrin-binding protein
• GBP
• HADH
• hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase(trifunctional protein), alpha subunit
• hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme Athiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
• LCEH
• LCHAD
• long-chain 2-enoyl-CoA hydratase
• long-chain-3-hydroxyacyl-CoA dehydrogenase
• MGC1728
• mitochondrial long-chain 2-enoyl-Coenzyme A (CoA) hydratase, alpha subunit
• mitochondrial long-chain L-3-hydroxyacyl-Coenzyme A (CoA) dehydrogenase, alphasubunit
• mitochondrial trifunctional enzyme, alpha subunit
• mitochondrial trifunctional protein, alpha subunit
• MTPATP-ALPHA
• TP-alpha
• trifunctional enzyme subunit alpha, mitochondrial

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HADHA Antibody (NBP1-83387)(1)

Publication using NBP1-83387

• Gerin I, Clerbaux LA, Haumont O et al. Expression of miR-33 from an SREBP2 intron inhibits cholesterol export and fatty acid oxidation. J Biol Chem 2010 Oct [PMID: 20732877]

Bioinformatics

• Gene Symbol: HADHA