P-Cadherin Antibody [Unconjugated]

Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-MouseP‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for P‑Cadherin at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

P‑Cadherin was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Mouse P‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to intercellular junctions. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

P‑Cadherin was detected in immersion fixed frozen sections of mouse embryo (15 dpc) using Goat Anti-MouseP‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue and lungs. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysates of mouse embryo tissue, loaded at 0.2 mg/mL. A specific band was detected for P‑Cadherin at approximately 115 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse P‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Mu
• Applications: WB, Simple Western, Flow, IHC, B/N, CyTOF-ready, ELISA(Cap), ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

P-Cadherin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse P-Cadherin
  Glu100-Gly647
  Accession # Q8BSL6
• Specificity: Detects P‑Cadherin in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant human (rh) P‑Cadherin is observed and less than 0.3% cross-reactivity with recombinant mouse E-Cadherin, rhN-Cadherin, and rhCadherin-8 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CDH3
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Adhesion Blockade
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 25 ug/mL
  • Western Blot 0.5 ug/mL
• Application Notes: ELISA Capture: Mouse P-Cadherin Antibody (Catalog # AF761)
  ELISA Detection: Mouse P-Cadherin Biotinylated Antibody (Catalog # BAF761)
  Standard: Recombinant Mouse P-Cadherin Fc Chimera (Catalog # 761-MP)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for P-Cadherin Antibody [Unconjugated]

• CAD3
• cadherin 3, P-cadherin (placental)
• cadherin 3, type 1, P-cadherin (placental)
• Cadherin-3
• CDH3
• CDHP
• CDHPcalcium-dependent adhesion protein, placental
• HJMD
• PCAD
• PCadherin
• P-Cadherin
• PCADP-cadherin
• Placental cadherin

Background

Placental Cadherin (P-Cadherin or PCAD) is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha , beta , and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Constitutive P-Cadherin expression is found in the epidermis, mesothelium, corneal epithelium, and uterine decidua. Mouse P-Cadherin is an 822 amino acid (aa) protein with a 27 aa signal sequence and a 795 aa propeptide. The mature protein begins at aa 100 and has a 542 aa extracellular region, a 27 aa transmembrane region, and a 153 aa cytoplasmic region.

1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
2. Overduin, M. et al. (1995) Science 267:386.
3. Takeichi, M. (1991) Science 251:1451.
4. Nose, A. et al. (1987) EMBO J. 6:3655.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P-Cadherin Antibody (AF761)(10)

Publications using AF761

• KJ Ng, J Lim, YN Tan, D Quek, Z Lim, N Pantelirei, C Clavel Sox2 in the dermal papilla regulates hair follicle pigmentation Oncogene, 2022;40(3):111100. 2022 [PMID: 35858560] (IHC, Mouse)
• S Choi, B Zhang, S Ma, M Gonzalez-C, D Stein, X Jin, ST Kim, YL Kang, A Besnard, A Rezza, L Grisanti, JD Buenrostro, M Rendl, M Nahrendorf, A Sahay, YC Hsu Corticosterone inhibits GAS6 to govern hair follicle stem-cell quiescence Nature, 2021;0(0):. 2021 [PMID: 33790465] (IHC, Mouse)
• S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing Cell Stem Cell, 2020;27(3):396-412.e6. 2020 [PMID: 32755548] (ICC, Mouse)
• S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing Cell Stem Cell, 2020;27(3):396-412.e6. 2020 [PMID: 32755548] (IHC, Transgenic Mouse)
• M Ge, C Liu, L Li, M Lan, Y Yu, L Gu, Y Su, K Zhang, Y Zhang, T Wang, C Liu, F Liu, M Li, L Xiong, K Wang, T He, Y Dai, Y Zhao, N Li, Z Yu, Q Meng miR-29a/b1 Inhibits Hair Follicle Stem Cell Lineage Progression by Spatiotemporally Suppressing WNT and BMP Signaling Cell Rep, 2019;29(8):2489-2504.e4. 2019 [PMID: 31747615] (IHC, Mouse)
• M Binder, P Chmielarz, PJ Mckinnon, LC Biggs, I Thesleff, A Balic Functionally Distinctive Ptch Receptors Establish Multimodal Hedgehog Signaling in the Tooth Epithelial Stem Cell Niche Stem Cells, 2019;0(0):. 2019 [PMID: 31145830] (IHC, Mouse)
• X Yang, JY Chung, U Rai, N Esumi Cadherins in the retinal pigment epithelium (RPE) revisited: P-cadherin is the highly dominant cadherin expressed in human and mouse RPE in vivo PLoS ONE, 2018;13(1):e0191279. 2018 [PMID: 29338041] (IHC, Mouse)
• JD Hoeck, B Biehs, AV Kurtova, NM Kljavin, F de Sousa E, B Alicke, H Koeppen, Z Modrusan, R Piskol, FJ de Sauvage Stem cell plasticity enables hair regeneration following Lgr5(+) cell loss Nat. Cell Biol., 2017;19(6):666-676. 2017 [PMID: 28553937] (IHC, Mouse)
• Y Ge, NC Gomez, RC Adam, M Nikolova, H Yang, A Verma, CP Lu, L Polak, S Yuan, O Elemento, E Fuchs Stem Cell Lineage Infidelity Drives Wound Repair and Cancer Cell, 2017;0(0):. 2017 [PMID: 28434617] (IHC, Mouse)
• Saint-Geniez M, Kurihara T, D'Amore PA Role of cell and matrix-bound VEGF isoforms in lens development. Invest. Ophthalmol. Vis. Sci., 2009;50(1):311-21. 2009 [PMID: 18757513] (IHC-Fr, Mouse)

Review for P-Cadherin Antibody (AF761) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous - IF Mouse 01/09/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 20826529
• Species: Mouse

Bioinformatics

• Gene Symbol: CDH3
• Uniprot:
  • Q8BSL6()