P-Cadherin Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-MouseP‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) followed by ...read more
P‑Cadherin was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Mouse P‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) at 10 µg/mL for 3 hours ...read more
P‑Cadherin was detected in immersion fixed frozen sections of mouse embryo (15 dpc) using Goat Anti-MouseP‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) at 15 µg/mL overnight at 4 °C. ...read more
Simple Western lane view shows lysates of mouse embryo tissue, loaded at 0.2 mg/mL. A specific band was detected for P‑Cadherin at approximately 115 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, B/N, CyTOF-ready, ELISA(Cap), ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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P-Cadherin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse P-Cadherin
Glu100-Gly647
Accession # Q8BSL6
Specificity
Detects P‑Cadherin in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant human (rh) P‑Cadherin is observed and less than 0.3% cross-reactivity with recombinant mouse E-Cadherin, rhN-Cadherin, and rhCadherin-8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CDH3
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Adhesion Blockade
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 25 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
ELISA Capture: Mouse P-Cadherin Antibody (Catalog # AF761)
ELISA Detection: Mouse P-Cadherin Biotinylated Antibody (Catalog # BAF761)
Standard: Recombinant Mouse P-Cadherin Fc Chimera (Catalog # 761-MP)
Reviewed Applications
Read 1 Review rated 3
using
AF761 in the following applications:

Publications
Read Publications using
AF761 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for P-Cadherin Antibody [Unconjugated]

  • CAD3
  • cadherin 3, P-cadherin (placental)
  • cadherin 3, type 1, P-cadherin (placental)
  • Cadherin-3
  • CDH3
  • CDHP
  • CDHPcalcium-dependent adhesion protein, placental
  • HJMD
  • PCAD
  • PCadherin
  • P-Cadherin
  • PCADP-cadherin
  • Placental cadherin

Background

Placental Cadherin (P-Cadherin or PCAD) is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha , beta , and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Constitutive P-Cadherin expression is found in the epidermis, mesothelium, corneal epithelium, and uterine decidua. Mouse P-Cadherin is an 822 amino acid (aa) protein with a 27 aa signal sequence and a 795 aa propeptide. The mature protein begins at aa 100 and has a 542 aa extracellular region, a 27 aa transmembrane region, and a 153 aa cytoplasmic region.

  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) Science 251:1451.
  4. Nose, A. et al. (1987) EMBO J. 6:3655.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for P-Cadherin Antibody (AF761)(10)

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 3 applications: ICC, IHC, IHC-Fr.


Filter By Application
ICC
(1)
IHC
(8)
IHC-Fr
(1)
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Mouse
(9)
Transgenic Mouse
(1)
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Showing Publications 1 - 10 of 10.
Publications using AF761 Applications Species
KJ Ng, J Lim, YN Tan, D Quek, Z Lim, N Pantelirei, C Clavel Sox2 in the dermal papilla regulates hair follicle pigmentation Oncogene, 2022;40(3):111100. 2022 [PMID: 35858560] (IHC, Mouse) IHC Mouse
S Choi, B Zhang, S Ma, M Gonzalez-C, D Stein, X Jin, ST Kim, YL Kang, A Besnard, A Rezza, L Grisanti, JD Buenrostro, M Rendl, M Nahrendorf, A Sahay, YC Hsu Corticosterone inhibits GAS6 to govern hair follicle stem-cell quiescence Nature, 2021;0(0):. 2021 [PMID: 33790465] (IHC, Mouse) IHC Mouse
S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing Cell Stem Cell, 2020;27(3):396-412.e6. 2020 [PMID: 32755548] (ICC, Mouse) ICC Mouse
S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing Cell Stem Cell, 2020;27(3):396-412.e6. 2020 [PMID: 32755548] (IHC, Transgenic Mouse) IHC Transgenic Mouse
M Ge, C Liu, L Li, M Lan, Y Yu, L Gu, Y Su, K Zhang, Y Zhang, T Wang, C Liu, F Liu, M Li, L Xiong, K Wang, T He, Y Dai, Y Zhao, N Li, Z Yu, Q Meng miR-29a/b1 Inhibits Hair Follicle Stem Cell Lineage Progression by Spatiotemporally Suppressing WNT and BMP Signaling Cell Rep, 2019;29(8):2489-2504.e4. 2019 [PMID: 31747615] (IHC, Mouse) IHC Mouse
M Binder, P Chmielarz, PJ Mckinnon, LC Biggs, I Thesleff, A Balic Functionally Distinctive Ptch Receptors Establish Multimodal Hedgehog Signaling in the Tooth Epithelial Stem Cell Niche Stem Cells, 2019;0(0):. 2019 [PMID: 31145830] (IHC, Mouse) IHC Mouse
X Yang, JY Chung, U Rai, N Esumi Cadherins in the retinal pigment epithelium (RPE) revisited: P-cadherin is the highly dominant cadherin expressed in human and mouse RPE in vivo PLoS ONE, 2018;13(1):e0191279. 2018 [PMID: 29338041] (IHC, Mouse) IHC Mouse
JD Hoeck, B Biehs, AV Kurtova, NM Kljavin, F de Sousa E, B Alicke, H Koeppen, Z Modrusan, R Piskol, FJ de Sauvage Stem cell plasticity enables hair regeneration following Lgr5(+) cell loss Nat. Cell Biol., 2017;19(6):666-676. 2017 [PMID: 28553937] (IHC, Mouse) IHC Mouse
Y Ge, NC Gomez, RC Adam, M Nikolova, H Yang, A Verma, CP Lu, L Polak, S Yuan, O Elemento, E Fuchs Stem Cell Lineage Infidelity Drives Wound Repair and Cancer Cell, 2017;0(0):. 2017 [PMID: 28434617] (IHC, Mouse) IHC Mouse
Saint-Geniez M, Kurihara T, D'Amore PA Role of cell and matrix-bound VEGF isoforms in lens development. Invest. Ophthalmol. Vis. Sci., 2009;50(1):311-21. 2009 [PMID: 18757513] (IHC-Fr, Mouse) IHC-Fr Mouse

Review for P-Cadherin Antibody (AF761) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF761:
Filter by Applications
IF
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
  3
reviewed by:
Anonymous -
IF Mouse 01/09/2015
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Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 20826529
SpeciesMouse

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Recent Reviews

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Anonymous -
01/09/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol CDH3
Uniprot