P-Cadherin Antibody [Unconjugated]

Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse P-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for P-Cadherin at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

P-Cadherin was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Mouse P-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; NL003) and counterstained with DAPI (blue). Specific staining was localized to intercellular junctions. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

P-Cadherin was detected in immersion fixed frozen sections of mouse embryo (15 dpc) using Goat Anti-Mouse P-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue and lungs. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

P‑Cadherin was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Mouse P‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) at 0.5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of mouse embryo tissue, loaded at 0.2 mg/mL. A specific band was detected for P-Cadherin at approximately 115 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse P-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

BMPR1a regulated P-cadherin expression via p63 and Slug. (A) Immunohistochemistry staining for P-cadherin in control (n = 4 mice) and cKO (n = 3 mice) mammary glands at pregnancy day 14.5. Scale bar, 50 μm. (B) Western blotting for P-cadherin in mammary epithelial cells isolated from control and cKO mice at pregnancy day 14.5. beta -Tubulin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta -Tubulin. n = 3 mice. (C) qRT-PCR analysis of Cdh3 in FACS-sorted control and cKO myoepithelial cells at pregnancy day 14.5. n = 4 biological replicates. (D) P-cadherin (red) and K14 (green) double immunofluorescence staining in HC11 mammary epithelial cells treated with BMP4 (50 ng/mL) for 24 h. n = 3 biological replicates. Scale bar, 25 μm. (E) Western blotting for P-cadherin in HC11 mammary epithelial cells treated with BMP4 (50 ng/mL) for 24 h. beta -Tubulin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta -Tubulin. n = 3 biological replicates. (F) Scatter plot showing the correlation between BMPR1A and CDH3 expression in mammary glands from TCGA and GTEx data. Pearson’s coefficient test was performed to assess statistical significance. (G) Western blotting for P-cadherin in HC11 cells treated with p63 siRNA (sip63)/Slug siRNA (siSlug) and scramble RNA (NC) at 48 h. beta -Actin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta -Actin. n = 3 biological replicates. (H) Immunofluorescence for P-cadherin (green) in HC11 cells treated with p63 siRNA (sip63)/Slug siRNA (siSlug) and scramble RNA (NC) at 48 h. n = 3 biological replicates. Scale bar, 25 μm. (I) Western blotting for K14, P-cadherin, p63 and Slug in HC11 cells treated with scramble RNA, p63 siRNA, Slug siRNA, or p63 siRNA and Slug siRNA at 48 h. beta -Actin was used as a loading control. Statistical analysis the expression of K14/ beta -Actin, P-cadherin/ beta -Actin, p63/ beta -Actin and Slug/ beta -Actin. n = 3 biological replicates. Data were presented as means ± SD. ∗p 0.05 by unpaired two-tailed Student’s t-test). (D) Representative pictures of P5 pups’ mouths demonstrating the growth of the teeth (black arrows). (E) Images of P5 tongue sagittal sections immunolabeled for METTL3 and PCAD demonstrating the tongue surface of the Mettl3 cKO animals tends to be smoother than that of the control animals, which suggests filiform papillae are not well-formed in the cKO animals (scale bars: 50 µm). (F) Hematoxylin and eosin (H and E) stained P6 back skin sagittal sections (scale bars: 100 µm).Figure 2—figure supplement 2—source data 1.Quantification of neonates' body weights in (B).Figure 2—figure supplement 2—source data 2.Quantification of TEWL in (C).Quantification of neonates' body weights in (B).Quantification of TEWL in (C). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32845239), licensed under a CC-BY license. Not internally tested by R&D Systems.

Krt14-Cre driven conditional Mettl3 knockout mice display severe defects in HF morphogenesis. (B) Confocal images of E16.5 whole-mount back skin immunolabeled for P-cadherin (PCAD), METTL3&YFP (scale bars: 20 µm). Note that nuclear METTL3 immunofluorescence is selectively depleted from the YFP+ cells in cKO skin. White dashed lines denote the dermal-epidermal border. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32845239), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, Simple Western, Flow, IHC, B/N, CyTOF-ready, ELISA(Cap), ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

P-Cadherin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse P-Cadherin
  Glu100-Gly647
  Accession # Q8BSL6
• Specificity: Detects P‑Cadherin in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant human (rh) P‑Cadherin is observed and less than 0.3% cross-reactivity with recombinant mouse E-Cadherin, rhN-Cadherin, and rhCadherin-8 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CDH3
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Adhesion Blockade
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 0.5-15 ug/mL
  • Simple Western 25 ug/mL
  • Western Blot 0.5 ug/mL
• Application Notes: ELISA Capture: Mouse P-Cadherin Antibody (Catalog # AF761)
  ELISA Detection: Mouse P-Cadherin Biotinylated Antibody (Catalog # BAF761)
  Standard: Recombinant Mouse P-Cadherin Fc Chimera (Catalog # 761-MP)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for P-Cadherin Antibody [Unconjugated]

• CAD3
• cadherin 3, P-cadherin (placental)
• cadherin 3, type 1, P-cadherin (placental)
• Cadherin-3
• CDH3
• CDHP
• CDHPcalcium-dependent adhesion protein, placental
• HJMD
• PCAD
• PCadherin
• P-Cadherin
• PCADP-cadherin
• Placental cadherin

Background

Placental Cadherin (P-Cadherin or PCAD) is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha , beta , and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Constitutive P-Cadherin expression is found in the epidermis, mesothelium, corneal epithelium, and uterine decidua. Mouse P-Cadherin is an 822 amino acid (aa) protein with a 27 aa signal sequence and a 795 aa propeptide. The mature protein begins at aa 100 and has a 542 aa extracellular region, a 27 aa transmembrane region, and a 153 aa cytoplasmic region.

1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
2. Overduin, M. et al. (1995) Science 267:386.
3. Takeichi, M. (1991) Science 251:1451.
4. Nose, A. et al. (1987) EMBO J. 6:3655.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P-Cadherin Antibody (AF761)(26)

Publications using AF761

• Luyang Xiong, Irina Zhevlakova, Xiaoxia Z. West, Detao Gao, Rakhylia Murtazina, Anthony Horak et al. TLR2 Regulates Hair Follicle Cycle and Regeneration via BMP Signaling bioRxiv 9/20/2023 [PMID: 37645905]
• Wang, X;Ramos, R;Phan, AQ;Yamaga, K;Flesher, JL;Jiang, S;Oh, JW;Jin, S;Jahid, S;Kuan, CH;Nguyen, TK;Liang, HY;Shettigar, NU;Hou, R;Tran, KH;Nguyen, A;Vu, KN;Phung, JL;Ingal, JP;Levitt, KM;Cao, X;Liu, Y;Deng, Z;Taguchi, N;Scarfone, VM;Wang, G;Paolilli, KN;Wang, X;Guerrero-Juarez, CF;Davis, RT;Greenberg, EN;Ruiz-Vega, R;Vasudeva, P;Murad, R;Widyastuti, LHP;Lee, HL;McElwee, KJ;Gadeau, AP;Lawson, DA;Andersen, B;Mortazavi, A;Yu, Z;Nie, Q;Kunisada, T;Karin, M;Tuckermann, J;Esko, JD;Ganesan, AK;Li, J;Plikus, MV; Signalling by senescent melanocytes hyperactivates hair growth Nature 2023-06-01 [PMID: 37344645] (Immunohistochemistry, Transgenic Mouse)
• Stewart, KS;Gonzales, KA;Yuan, S;Tierney, MT;Bonny, AR;Yang, Y;Infarinato, NR;Cowley, CJ;Levorse, JM;Pasolli, HA;Ghosh, S;Rothlin, CV;Fuchs, E; Stem cells tightly regulate dead cell clearance to maintain tissue fitness bioRxiv : the preprint server for biology 2023-05-22 [PMID: 37293114] (IHC, Mouse)
• A Dhingra, JW Tobias, NJ Philp, K Boesze-Bat Transcriptomic Changes Predict Metabolic Alterations in LC3 Associated Phagocytosis in Aged Mice International Journal of Molecular Sciences, 2023-04-04;24(7):. 2023-04-04 [PMID: 37047689] (IHC, Mouse)
• Seong Yeong An, Hyo-Sung Kim, So Yeon Kim, Se Young Van, Han Jun Kim, Jae-Hyung Lee et al. Keratin-mediated hair growth and its underlying biological mechanism Communications Biology 11/19/2022 [PMID: 36402892]
• Xuan Liang, Antonia Weberling, Chun Yuan Hii, Magdalena Zernicka‐Goetz, Clare E Buckley E‐cadherin mediates apical membrane initiation site localisation during de novo polarisation of epithelial cavities The EMBO Journal 12/15/2022 [PMID: 35993232]
• KJ Ng, J Lim, YN Tan, D Quek, Z Lim, N Pantelirei, C Clavel Sox2 in the dermal papilla regulates hair follicle pigmentation Oncogene, 2022-07-19;40(3):111100. 2022-07-19 [PMID: 35858560] (IHC, Mouse)
• Zumer Naeem, Sven Zukunft, Stephan Günther, Stefan Liebner, Andreas Weigert, Bruce D. Hammock et al. Role of the soluble epoxide hydrolase in the hair follicle stem cell homeostasis and hair growth Pflügers Archiv - European Journal of Physiology 9/1/2022 [PMID: 35648219]
• Qu R, Gupta K, Dong D et al. Decomposing a deterministic path to mesenchymal niche formation by two intersecting morphogen gradients Developmental cell 4/25/2022 [PMID: 35421372]
• S Choi, B Zhang, S Ma, M Gonzalez-C, D Stein, X Jin, ST Kim, YL Kang, A Besnard, A Rezza, L Grisanti, JD Buenrostro, M Rendl, M Nahrendorf, A Sahay, YC Hsu Corticosterone inhibits GAS6 to govern hair follicle stem-cell quiescence Nature, 2021-03-31;0(0):. 2021-03-31 [PMID: 33790465] (IHC, Mouse)
• Linghe Xi, Thomas Carroll, Irina Matos, Ji-Dung Luo, Lisa Polak, H Amalia Pasolli et al. m6A RNA methylation impacts fate choices during skin morphogenesis eLife 8/26/2020 [PMID: 32845239]
• S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing Cell Stem Cell, 2020-08-04;27(3):396-412.e6. 2020-08-04 [PMID: 32755548] (ICC, IHC, Mouse, Transgenic Mouse)
• Guo Li, Xiaolong Tang, Shuping Zhang, Meiling Jin, Ming Wang, Zhili Deng et al. SIRT 7 activates quiescent hair follicle stem cells to ensure hair growth in mice The EMBO Journal 9/15/2020 [PMID: 32696520]
• Irina Matos, Amma Asare, John Levorse, Tamara Ouspenskaia, June de la Cruz-Racelis, Laura-Nadine Schuhmacher et al. Progenitors oppositely polarize WNT activators and inhibitors to orchestrate tissue development eLife 4/20/2020 [PMID: 32310087]
• M Ge, C Liu, L Li, M Lan, Y Yu, L Gu, Y Su, K Zhang, Y Zhang, T Wang, C Liu, F Liu, M Li, L Xiong, K Wang, T He, Y Dai, Y Zhao, N Li, Z Yu, Q Meng miR-29a/b1 Inhibits Hair Follicle Stem Cell Lineage Progression by Spatiotemporally Suppressing WNT and BMP Signaling Cell Rep, 2019-11-19;29(8):2489-2504.e4. 2019-11-19 [PMID: 31747615] (IHC, Mouse)
• Melanie Laurin, Nicholas C Gomez, John Levorse, Ataman Sendoel, Megan Sribour, Elaine Fuchs An RNAi screen unravels the complexities of Rho GTPase networks in skin morphogenesis eLife 9/25/2019 [PMID: 31556874]
• S. Yamada, R. Lav, J. Li, A.S. Tucker, J.B.A. Green Molar Bud-to-Cap Transition Is Proliferation Independent Journal of Dental Research 10/1/2019 [PMID: 31393749]
• M Binder, P Chmielarz, PJ Mckinnon, LC Biggs, I Thesleff, A Balic Functionally Distinctive Ptch Receptors Establish Multimodal Hedgehog Signaling in the Tooth Epithelial Stem Cell Niche Stem Cells, 2019-06-10;0(0):. 2019-06-10 [PMID: 31145830] (IHC, Mouse)
• Gupta K, Levinsohn J, Linderman G et al. Single-Cell Analysis Reveals a Hair Follicle Dermal Niche Molecular Differentiation Trajectory that Begins Prior to Morphogenesis Dev. Cell 12/19/2018 [PMID: 30595533]
• X Yang, JY Chung, U Rai, N Esumi Cadherins in the retinal pigment epithelium (RPE) revisited: P-cadherin is the highly dominant cadherin expressed in human and mouse RPE in vivo PLoS ONE, 2018-01-16;13(1):e0191279. 2018-01-16 [PMID: 29338041] (IHC, Mouse)
• Audrey Shimei Wang, Peh Fern Ong, Alexandre Chojnowski, Carlos Clavel, Oliver Dreesen Loss of lamin B1 is a biomarker to quantify cellular senescence in photoaged skin Scientific Reports 11/15/2017 [PMID: 29142250]
• Samara Brown, Cristiana M. Pineda, Tianchi Xin, Jonathan Boucher, Kathleen C. Suozzi, Sangbum Park et al. Correction of aberrant growth preserves tissue homeostasis Nature 8/2/2017 [PMID: 28783732]
• JD Hoeck, B Biehs, AV Kurtova, NM Kljavin, F de Sousa E, B Alicke, H Koeppen, Z Modrusan, R Piskol, FJ de Sauvage Stem cell plasticity enables hair regeneration following Lgr5(+) cell loss Nat. Cell Biol., 2017-05-29;19(6):666-676. 2017-05-29 [PMID: 28553937] (IHC, Mouse)
• Chung-Ping Liao, Reid C. Booker, Sean J. Morrison, Lu Q. Le Identification of hair shaft progenitors that create a niche for hair pigmentation Genes & Development 4/15/2017 [PMID: 28465357]
• Y Ge, NC Gomez, RC Adam, M Nikolova, H Yang, A Verma, CP Lu, L Polak, S Yuan, O Elemento, E Fuchs Stem Cell Lineage Infidelity Drives Wound Repair and Cancer Cell, 2017-04-20;0(0):. 2017-04-20 [PMID: 28434617] (IHC, Mouse)
• Saint-Geniez M, Kurihara T, D'Amore PA Role of cell and matrix-bound VEGF isoforms in lens development. Invest. Ophthalmol. Vis. Sci., 2008-08-29;50(1):311-21. 2008-08-29 [PMID: 18757513] (IHC-Fr, Mouse)

Review for P-Cadherin Antibody (AF761) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IF Mouse 01/09/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 20826529
• Species: Mouse

Bioinformatics

• Gene Symbol: CDH3
• Uniprot:
  • Q8BSL6()