Lysyl Oxidase Homolog 2/LOXL2 Antibody (5D2) - Azide and BSA Free

Sandwich ELISA: Lysyl Oxidase Homolog 2/LOXL2 Antibody (5D2) [H00004017-M01] - Detection limit for recombinant GST tagged LOXL2 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 5D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Lysyl Oxidase Homolog 2/LOXL2 Antibody (5D2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: LOXL2 (NP_002309, 675 a.a. ~ 773 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NFGDQGITMGCWDMYRHDIDCQWVDITDVPPGDYLFQVVINPNFEVAESDYSNNIMKCRSRYDGHRIWMYNCHIGGSFSEETEKKFEHFSGLLNNQLSP
• Localization: Secreted, extracellular space.
• Specificity: LOXL2 - lysyl oxidase-like 2
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LOXL2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Lysyl Oxidase Homolog 2/LOXL2 Antibody (5D2) - Azide and BSA Free

• EC 1.4.3
• EC 1.4.3.-
• LOL2
• LOR2
• LOXL2
• Lysyl Oxidase Homolog 2
• lysyl oxidase related 2
• lysyl oxidase-like 2
• Lysyl oxidase-like protein 2
• Lysyl oxidase-related protein 2
• Lysyl oxidase-related protein WS9-14
• WS9-14

Background

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lysyl Oxidase Homolog 2/LOXL2 Antibody (H00004017-M01)(6)

Publications using H00004017-M01

• Umana-Diaz C, Pichol-Thievend C, Marchand M et al. Scavenger Receptor Cysteine-Rich domains of Lysyl Oxidase-Like2 regulate endothelial ECM and angiogenesis through non-catalytic scaffolding mechanisms. Matrix Biol. 2019-11-20 [PMID: 31759052]
• Schmelzer C, Heinz A, Troilo H et al. Lysyl oxidase-like 2 (LOXL2)-mediated cross-linking of tropoelastin. FASEB J. 2019-01-24 [PMID: 30676771]
• Zhan XH, Jiao JW, Zhang HF et al. A three-gene signature from protein-protein interaction network of LOXL2- and actin-related proteins for esophageal squamous cell carcinoma prognosis. Cancer Med 2017-05-29 [PMID: 28556501]
• Li TY, Xu LY, Wu ZY et al. Reduced nuclear and ectopic cytoplasmic expression of lysyl oxidase-like 2 is associated with lymph node metastasis and poor prognosis in esophageal squamous cell carcinoma. Hum Pathol. 2011-12-26 [PMID: 22204712]
• Li T, Li D, Cheng L et al. Epithelial-mesenchymal transition induced by hepatitis C virus core protein in cholangiocarcinoma. Ann Surg Oncol 17(7):1937-44. 2010-07-01 [PMID: 20162464]
• Fujimoto E, Tajima S. Reciprocal regulation of LOX and LOXL2 expression during cell adhesion and terminal differentiation in epidermal keratinocytes. J Dermatol Sci. 2009-04-23 [PMID: 19394199]

Bioinformatics

• Gene Symbol: LOXL2
• Entrez:
  • 4017(Human)
• OMIM:
  • 606663(Human)
• Uniprot:
  • NP_002309(Human)