Lumican Antibody [Unconjugated]

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Lumican was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Lumican Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2745) at 15 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Lumican Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Lumican
Gln19-Asn338, predicted
Accession # P51885
Specificity
Detects mouse Lumican in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LUM
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2745 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lumican Antibody [Unconjugated]

  • KSPG lumican
  • LDC
  • LUM
  • lumican proteoglycan
  • Lumican
  • SLRR2D
  • SLRR2DLDCKeratan sulfate proteoglycan lumican

Background

Lumican is a 40 kDa member of the family of small leucine-rich repeat proteoglycans (SLRPs) and the class II subfamily (1). Mouse Lumican is synthesized as a 338 amino acid (aa) precursor that contains an 18 aa signal sequence and a 320 aa mature chain (SwissProt #: 51885). The mature chain contains a negatively charged N‑terminal domain containing sulfated tyrosine and disulfide bonds, 12 leucine-rich repeats (LRRs), four potential sites of N-linked glycosylation, and a carboxyl terminal domain containing two conserved cysteines (1). Mature mouse Lumican is 98%, 88%, and 70% aa identical to mature rat, human, and chick Lumican, respectively. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins (ECM) proteins (1-2). Lumican is expressed in a variety of tissues, including skin, artery, lung, cornea, kidney, bone, aorta, and articular cartilage (1). Lumican’s role in vivo has been found using Lumican null mice. These mice have functional deficits including corneal opacity as well as skin and tendon fragility associated with disorganized and loosely packed collagen fibers (1, 3-6). The abnormal connective tissue phenotype seen in the Lumican null mice shows the importance of the role of Lumican in collagen fibrillogenesis (1). In addition to the control of collagen fibril assembly, Lumican has been shown to play a role in the regulation of cell proliferation (7-8), migration (8-9), and adhesion (9). Lumican’s overexpression has been reported in carcinoid tumor, breast, colorectal, neuroendocrine, uterine cervical, and pancreatic cancers (10).
  1. Nikitovic, D. et al. (2008) IUBMB Life 60:818.
  2. Blochberger, T.C. et al. (1992) J. Biol. Chem. 267:347.
  3. Chakravarti, S. et al. (1998) J. Cell Biol. 141:1277.
  4. Chakravarti, S. et al. (2000) Invest. Ophthalmol. Vis. Sci. 41:3365.
  5. Jepsen, K.J. et al. (2002) J. Biol. Chem. 277:35532.
  6. Chakravarti, S. et al. (2003) Invest. Ophthalmol. Vis. Sci. 44:2422.
  7. Vuillermoz, B. et al. (2004) Exp. Cell Res. 296:294
  8. Nikitovic, D. et al. (2008) FEBS J. 275:350.
  9. D’Onofrio, M.F. et al. (2008) Biochem. Biophys. Res. Commun. 365:266.
  10. Ishiwata, T. et al. (2007) Oncol. Rep. 18:537.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lumican Antibody (AF2745)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: IHC, IHC-P, Western Blot.


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IHC
(2)
IHC-P
(2)
Western Blot
(3)
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Mouse
(7)
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Showing Publications 1 - 7 of 7.
Publications using AF2745 Applications Species
AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases Sci Rep, 2020;10(1):12912. 2020 [PMID: 32737331] (Western Blot, Mouse) Western Blot Mouse
HW Cheng, L Onder, M Novkovic, C Soneson, M Lütge, N Pikor, E Scandella, MD Robinson, JI Miyazaki, A Tersteegen, U Sorg, K Pfeffer, T Rülicke, T Hehlgans, B Ludewig Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp Nat Commun, 2019;10(1):1739. 2019 [PMID: 30988302] (IHC, Mouse) IHC Mouse
G Wolff, AE Taranko, I Meln, J Weinmann, T Sijmonsma, S Lerch, D Heide, AT Billeter, D Tews, D Krunic, P Fischer-Po, BP Müller-Sti, S Herzig, D Grimm, M Heikenwäld, WW Kao, A Vegiopoulo Diet-dependent function of the extracellular matrix proteoglycan Lumican in obesity and glucose homeostasis Mol Metab, 2018;0(0):. 2018 [PMID: 30409703] (Western Blot, Mouse) Western Blot Mouse
Wang L, Foster B, Kram V, Nociti F, Zerfas P, Tran A, Young M, Somerman M Fibromodulin and Biglycan Modulate Periodontium through TGFbeta/BMP Signaling. J Dent Res, 2014;93(8):780-787. 2014 [PMID: 24966230] (IHC-P, Mouse) IHC-P Mouse
Krishnan A, Li X, Kao W, Viker K, Butters K, Masuoka H, Knudsen B, Gores G, Charlton M Lumican, an extracellular matrix proteoglycan, is a novel requisite for hepatic fibrosis. Lab Invest, 2012;92(12):1712-25. 2012 [PMID: 23007134] (Western Blot, Mouse) Western Blot Mouse
Salgado RM, Favaro RR, Zorn TM Modulation of small leucine-rich proteoglycans (SLRPs) expression in the mouse uterus by estradiol and progesterone. Reprod. Biol. Endocrinol., 2011;9(1):22. 2011 [PMID: 21294898] (IHC-P, Mouse) IHC-P Mouse
Salgado RM, Favaro RR, Martin SS, Zorn TM The estrous cycle modulates small leucine-rich proteoglycans expression in mouse uterine tissues. Anat Rec (Hoboken), 2009;292(1):138-53. 2009 [PMID: 18951514] (IHC, Mouse) IHC Mouse

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Bioinformatics

Gene Symbol LUM
Uniprot