LRG1 Antibody (7A1) - BSA Free

Immunohistochemistry: LRG1 Antibody (7A1) [NBP3-26164] - Image of LRG1 Antibody (7A1) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot: LRG1 Antibody (7A1) [NBP3-26164] - Positive Western Blot detected in: L02 whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate. All lanes: LRG1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 39 kDa Observed band size: 39 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 7A1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

LRG1 Antibody (7A1) - BSA Free Summary

• Description: Novus Biologicals Rabbit LRG1 Antibody (7A1) - BSA Free (NBP3-26164) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human LRG1 [UniProt P02750]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LRG1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for LRG1 Antibody (7A1) - BSA Free

• 1300008B03Rik
• 2310031E04Rik
• HMFT1766
• leucine-rich alpha-2-glycoprotein 1
• leucine-rich alpha-2-glycoprotein
• LRG1
• LRGleucine rich alpha 2 glycoprotein

Background

The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation (O'Donnell et al., 2002 [PubMed 12223515]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LRG1