LRRC32/GARP Antibody

Western Blot: LRRC32/GARP Antibody [NBP2-24664] - Analysis of GARP protein expression; (t test, * P < 0.05). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31198639/) licensed under a CC-BY licence.

Flow Cytometry: LRRC32/GARP Antibody [NBP2-24664] - BALB/c splenocytes stained with anti-GARP (NBP2-24664), with a FITC conjugated secondary (20302), and anti-mouse CD4 PE (IMG-5922D). Viable cells in the lymphocyte gate were used for analysis. Novus's cell surface flow kit (10084K) was used for this test .

Western Blot: LRRC32/GARP Antibody [NBP2-24664] - Analysis of GARP/LRRC32 in mouse thymus lysate in the (1) absence and (2) presence of immunizing peptide using this antibody. 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

LRRC32/GARP Antibody Summary

• Immunogen: A portion of amino acids 250-300 of human GARP/LRRC32 was used as the immunogen.
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LRRC32
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-3 ug/ml
  • Western Blot 0.5-1.0 ug/ml

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31198639).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 ml PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for LRRC32/GARP Antibody

• D11S833E
• D11S833Eleucine-rich repeat-containing protein 32
• GARP
• GARPGarpin
• Garpin
• Glycoprotein A repetitions predominantgarpin
• leucine rich repeat containing 32
• LRRC32

Background

GARP, also known as leucine rich repeat containing 32, is a type I membrane protein with twenty-two leucine-rich repeats and a short intracytoplasmic region. GARP was identified based on the analysis of gene expression profiling of Treg and Th cells following TCR stimulation. Since GARP is expressed in CD4+, CD25+, FoxP3+ cells (Tregs), it may be a candidate for cell surface marker for Treg cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRRC32/GARP Antibody (NBP2-24664)(2)

Publications using NBP2-24664

• Li R, Sun J, Yang F et al. Effect of GARP on osteogenic differentiation of bone marrow mesenchymal stem cells via the regulation of TGF beta 1 in vitro PeerJ 2019-05-23 [PMID: 31198639] (FLOW, Rat)
• Macaulay IC, Thon JN, Tijssen MR et al. Canonical Wnt signaling in megakaryocytes regulates proplatelet formation. Blood. 2013-01-03 [PMID: 23160460] (WB, Human)
  
  Details:
  WB: Fig 3C (CHRF 288-11 human megarkeryocytic cell line), GARP/LRRC3 was detected at 72 kDA.

Bioinformatics

• Gene Symbol: LRRC32
• Uniprot:
  • Q14392()