LIS1 Antibody - BSA Free

Western Blot: LIS1 Antibody [NBP1-76815] - HeLa cell lysate with LIS1 antibody at (A) 0.5 and (B) 1 ug/mL.

Immunocytochemistry/ Immunofluorescence: LIS1 Antibody - BSA Free [NBP1-76815] - Immunocytochemistry of LIS1 in Jurkat cells with LIS1 antibody at 2.5 u/mL.

Immunocytochemistry/ Immunofluorescence: LIS1 Antibody - BSA Free [NBP1-76815] - Immunofluorescence of LIS1 in Jurkat cells with LIS1 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

LIS1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit LIS1 Antibody - BSA Free (NBP1-76815) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human LIS1. The immunogen is located within amino acids 340 - 390 of LIS1. Amino Acid Squence: DDKTLRVWDYKNKR
• Predicted Species: Porcine (100%), Bovine (100%), Chicken (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAFAH1B1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 2.5-20 ug/mL
  • Western Blot 0.5-1 ug/ml
• Control Peptide:

  LIS1 Peptide (NBP1-76815PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for LIS1 Antibody - BSA Free

• LIS-1
• LIS1PAF-AH alpha
• LIS2
• lissencephaly 1 protein
• Lissencephaly-1 protein
• MDCR
• MDS
• Miller-Dieker syndrome chromosome region
• PAF acetylhydrolase 45 kDa subunit
• PAF-AH 45 kDa subunit
• PAFAH alpha
• PAFAH
• PAFAHA
• platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)
• platelet-activating factor acetylhydrolase IB subunit alpha
• platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD)
• platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa
• platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa)

Background

Lissencephaly is a severe brain developmental disease characterized by the mislocalization of cortical neurons, a smooth cerebral surface, mental retardation, and seizures. Classical lissencephaly is caused by sporadic mutations in the LIS1 gene. While LIS1 is known to act in a pathway deactivating the lipid messenger platelet-activating factor, LIS1 forms a complex with Nudel and 14-3-3epsilon which is then transported from neuronal cell bodies through the actions of DISC1 and KIF5A, a microtubule-dependent directed motor protein kinesin. Decreased expression of LIS1 blocked neural stem cell division, morphogenesis, and motility, suggesting that LIS1 plays an important role in neuronal cell proliferation and localization in the developing brain. At least two isoforms of LIS1 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAFAH1B1
• Uniprot:
  • P43034(Human)