Lipocalin-2/NGAL Antibody [Unconjugated]

Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line and human pancreas tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Lipocalin‑2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of mouse and rat bone marrow. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Lipocalin‑2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Lipocalin‑2/NGAL was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human/Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Lipocalin‑2/NGAL was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human/Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lipocalin‑2/NGAL at approximately 34 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Lipocalin-2/NGAL Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived Recombinant Human Lipocalin‑2/NGAL
  Gln21-Gly198
  Accession # P80188
• Specificity: Detects human Lipocalin-2/NGAL in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LCN2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipocalin-2/NGAL Antibody [Unconjugated]

• 24p3
• 25 kDa alpha-2-microglobulin-related subunit of MMP-9
• HNL
• LCN2
• lipocalin 2 (oncogene 24p3)
• lipocalin 2
• Lipocalin2
• Lipocalin-2
• migration-stimulating factor inhibitor
• MSFI
• neutrophil gelatinase-associated lipocalin
• NGAL
• NGALlipocalin-2
• Oncogene 24p3
• p25
• Siderocalin
• Uterocalin

Background

Members of Lipocalin family share a highly conserved fold with an eight-stranded antiparallel beta barrel, and act as a transporters, carrying small molecules to specific cells. Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules. It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation. Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis. Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (K_d = 0.41 nM). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less K_d values (7.9 nM). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron. Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lipocalin-2/NGAL Antibody (AF1757)(22)

Publications using AF1757

• SL Kim, MW Shin, SW Kim Lipocalin 2 activates the NLRP3 inflammasome via LPS?induced NF?kappaB signaling and plays a role as a pro?inflammatory regulator in murine macrophages Molecular Medicine Reports, 2022;26(6):. 2022-01-01 [PMID: 36263611] (Western Blot, Mouse)
• SL Kim, MW Shin, SY Seo, SW Kim Lipocalin 2 potentially contributes to tumorigenesis from colitis via IL-6/STAT3/NF-kB signaling pathway Bioscience Reports, 2022;0(0):. 2022-01-01 [PMID: 35470375] (Western Blot, Human)
• L Cook, M Sengelmann, B Winkler, C Nagl, S Koch, U Schlomann, EP Slater, MA Miller, EP von Strand, B Dörsam, C Preu beta er, JW Bartsch ADAM8-Dependent Extracellular Signaling in the Tumor Microenvironment Involves Regulated Release of Lipocalin 2 and MMP-9 International Journal of Molecular Sciences, 2022;23(4):. 2022-01-01 [PMID: 35216088] (Western Blot, Human)
• F Yao, Y Deng, Y Zhao, Y Mei, Y Zhang, X Liu, C Martinez, X Su, RR Rosato, H Teng, Q Hang, S Yap, D Chen, Y Wang, MM Chen, M Zhang, H Liang, D Xie, X Chen, H Zhu, JC Chang, MJ You, Y Sun, B Gan, L Ma A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis Nature Communications, 2021;12(1):7333. 2021-01-01 [PMID: 34921145] (Western Blot, Human)
• AR Schiazza, EG Considine, M Betcher, BD Shepard Loss of renal olfactory receptor 1393 leads to improved glucose homeostasis in a type 1 diabetic mouse model Physiological Reports, 2021;9(23):e15007. 2021-01-01 [PMID: 34877823] (Western Blot, Mouse)
• GS Santiago-S, R Noriega-Ri, E Hernández-, F Valiyeva, B Quiñones-D, ES Villodre, BG Debeb, A Rosado-Alb, PE Vivas-Mejí Targeting Lipocalin-2 in Inflammatory Breast Cancer Cells with Small Interference RNA and Small Molecule Inhibitors International Journal of Molecular Sciences, 2021;22(16):. 2021-01-01 [PMID: 34445288] (Western Blot, Human)
• J Brockhaus, RB Craveiro, I Azraq, C Niederau, SK Schröder, R Weiskirche, J Jankowski, M Wolf In Vitro Compression Model for Orthodontic Tooth Movement Modulates Human Periodontal Ligament Fibroblast Proliferation, Apoptosis and Cell Cycle Biomolecules, 2021;11(7):. 2021-01-01 [PMID: 34201602] (Western Blot, Human)
• M Bannier-Hé, Y Post, J Korving, M Trani Bust, H Gehart, H Begthel, YE Bar-Ephrai, J van der Va, R Kalmann, SM Imhoff, H Clevers Exploring the human lacrimal gland using organoids and single-cell sequencing Cell Stem Cell, 2021;0(0):. 2021-01-01 [PMID: 33730555] (IHC, Human)
• B Bauvois, E Pramil, L Jondrevill, E Chapiro, C Quiney, K Maloum, SA Susin, F Nguyen-Kha Relation of Neutrophil Gelatinase-Associated Lipocalin Overexpression to the Resistance to Apoptosis of Tumor B Cells in Chronic Lymphocytic Leukemia Cancers (Basel), 2020;12(8):. 2020-01-01 [PMID: 32751884] (Western Blot, Human)
• SK Schröder, A Asimakopou, S Tillmann, S Koschmiede, R Weiskirche TNF-&alpha controls Lipocalin-2 expression in PC-3 prostate cancer cells Cytokine, 2020;135(0):155214. 2020-01-01 [PMID: 32712458] (ICC, Human)
• L Yammine, A Zablocki, W Baron, F Terzi, M Gallazzini Lipocalin-2 Regulates Epidermal Growth Factor Receptor Intracellular Trafficking Cell Rep, 2019;29(7):2067-2077.e6. 2019-01-01 [PMID: 31722218] (IP, Human)
• M Miki, T Oono, N Fujimori, T Takaoka, K Kawabe, Y Miyasaka, T Ohtsuka, D Saito, M Nakamura, Y Ohkawa, Y Oda, M Suyama, T Ito, Y Ogawa CLEC3A, MMP7, and LCN2 as novel markers for predicting recurrence in resected G1 and G2 pancreatic neuroendocrine tumors Cancer Med, 2019;0(0):. 2019-01-01 [PMID: 31129920] (IHC-P, Human)
• IT Myszor, Z Parveen, H Ottosson, P Bergman, B Agerberth, R Strömberg, GH Gudmundsso Novel aroylated phenylenediamine compounds enhance antimicrobial defense and maintain airway epithelial barrier integrity Sci Rep, 2019;9(1):7114. 2019-01-01 [PMID: 31068616] (Western Blot, Human)
• Z Wang, J Zhang, L Wang, W Li, L Chen, J Li, D Zhao, H Zhang, X Guo Glycine mitigates renal oxidative stress by suppressing Nox4 expression in rats with streptozotocin-induced diabetes J. Pharmacol. Sci., 2018;137(4):387-394. 2018-01-01 [PMID: 30196019] (Western Blot, Rat)
• K Ramani, CV Jawale, AH Verma, BM Coleman, JK Kolls, PS Biswas Unexpected kidney-restricted role for IL-17 receptor signaling in defense against systemic Candida albicans infection JCI Insight, 2018;3(9):. 2018-01-01 [PMID: 29720566] (IHC-P, Mouse)
• YS Park, Z Jin, EA Jeong, CO Yi, JY Lee, IS Park, GS Roh Cilostazol attenuates kainic acid-induced hippocampal cell death Korean J. Physiol. Pharmacol., 2018;22(1):63-70. 2018-01-01 [PMID: 29302213] (Western Blot, Mouse)
• YT Kang, WC Hsu, CH Wu, IL Hsin, PR Wu, KT Yeh, JL Ko Metformin alleviates nickel-induced autophagy and apoptosis via inhibition of hexokinase-2, activating lipocalin-2, in human bronchial epithelial cells Oncotarget, 2017;8(62):105536-105552. 2017-01-01 [PMID: 29285270] (Western Blot, Human)
• Shingo Haneda Expression of uterine lipocalin 2 and its receptor during early- to mid-pregnancy period in mares J. Reprod. Dev, 2016;0(0):. 2016-01-01 [PMID: 27980236] (Western Blot, Equine)
• The adipokine lipocalin-2 in the context of the osteoarthritic osteochondral junction Sci Rep, 2016;6(0):29243. 2016-01-01 [PMID: 27385438] (Western Blot, Human)
• Leung , Lisa, Radulovich , Nikolina, Zhu , Chang-Qi, Organ , Shawna, Bandarchi , Bizhan, Pintilie , Melania, To , Christin, Panchal , Devang, Tsao , Ming Sou Lipocalin2 promotes invasion, tumorigenicity and gemcitabine resistance in pancreatic ductal adenocarcinoma. PLoS ONE, 2012;7(10):e46677. 2012-01-01 [PMID: 23056397] (Western Blot, Human)
• Wortmann A, He Y, Christensen ME, Linn M, Lumley JW, Pollock PM, Waterhouse NJ, Hooper JD Cellular settings mediating Src Substrate switching between focal adhesion kinase tyrosine 861 and CUB-domain-containing protein 1 (CDCP1) tyrosine 734. J. Biol. Chem., 2011;286(49):42303-15. 2011-01-01 [PMID: 21994943] (Western Blot, Human)
• Kuwabara T, Mori K, Mukoyama M, Kasahara M, Yokoi H, Saito Y, Yoshioka T, Ogawa Y, Imamaki H, Kusakabe T, Ebihara K, Omata M, Satoh N, Sugawara A, Barasch J, Nakao K Urinary neutrophil gelatinase-associated lipocalin levels reflect damage to glomeruli, proximal tubules, and distal nephrons. Kidney Int., 2009;75(3):285-94. 2009-01-01 [PMID: 19148153] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: LCN2
• Uniprot:
  • P80188()