Lipocalin-2/NGAL Antibody (220310) [Unconjugated]

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Lipocalin‑2/NGAL Monoclonal Antibody (Catalog # MAB1757) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin‑2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Lipocalin‑2/NGAL was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human Lipocalin‑2/NGAL Monoclonal Antibody (Catalog # MAB1757) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane of ductal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line, HT‑29 human colon adenocarcinoma cell line, and human bone marrow tissue. PVDF membrane was probed with 0.2 µg/mL of Rat Anti-Human Lipocalin‑2/NGAL Monoclonal Antibody (Catalog # MAB1757) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin‑2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 220310
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Lipocalin-2/NGAL Antibody (220310) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Lipocalin-2/NGAL
  Gln21-Gly198
  Accession # P80188
• Specificity: Detects human Lipocalin-2/NGAL in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human (rh) Lipocalin-1 or rmLipocalin-2. This antibody also recognizes human Lipocalin‑2/MMP‑9 complexes in Western blots under non-reducing conditions.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: LCN2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.2-1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipocalin-2/NGAL Antibody (220310) [Unconjugated]

• 24p3
• 25 kDa alpha-2-microglobulin-related subunit of MMP-9
• HNL
• LCN2
• lipocalin 2 (oncogene 24p3)
• lipocalin 2
• Lipocalin2
• Lipocalin-2
• migration-stimulating factor inhibitor
• MSFI
• neutrophil gelatinase-associated lipocalin
• NGAL
• NGALlipocalin-2
• Oncogene 24p3
• p25
• Siderocalin
• Uterocalin

Background

Members of Lipocalin family share a highly conserved fold with an eight-stranded antiparallel beta barrel, and act as a transporters, carrying small molecules to specific cells (1). Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules (2). It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9) (2). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation (3). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3-5). Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (K_d = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less K_d values (7.9 nM) (6). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).

1. Flower, D.R. et al. (1994) FEBS Lett. 354:7.
2. Kjeldsen, L. et al. (1993) J. Biol. Chem. 268:10426.
3. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
4. Devireddy, L.R. et al. (2001) Science 293:829.
5. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
6. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
7. Flo, T.H. et al. (2004) Nature 432:917.
8. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lipocalin-2/NGAL Antibody (MAB1757)(11)

Publications using MAB1757

• F Lunardi, DI Abbrescia, L Vedovelli, F Pezzuto, F Fortarezza, GM Comacchio, V Guzzardo, P Ferrigno, M Loy, C Giraudo, AS Fraia, E Faccioli, F Braccioni, E Cozzi, D Gregori, GM Verleden, F Calabrese, FP Schena, F Rea Molecular Profiling of Tissue Samples with Chronic Rejection from Patients with Chronic Lung Allograft Dysfunction: A Pilot Study in Cystic Fibrosis Patients Biomolecules, 2023;13(1):. 2023-01-01 [PMID: 36671482] (IHC, Human)
• F Yao, Y Deng, Y Zhao, Y Mei, Y Zhang, X Liu, C Martinez, X Su, RR Rosato, H Teng, Q Hang, S Yap, D Chen, Y Wang, MM Chen, M Zhang, H Liang, D Xie, X Chen, H Zhu, JC Chang, MJ You, Y Sun, B Gan, L Ma A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis Nature Communications, 2021;12(1):7333. 2021-01-01 [PMID: 34921145] (Neutralization, Mouse)
• I Zhang, P Lépine, C Han, M Lacalle-Au, CX Chen, R Haag, TM Durcan, D Maysinger Nanotherapeutic Modulation of Human Neural Cells and Glioblastoma in Organoids and Monocultures Cells, 2020;9(11):. 2020-01-01 [PMID: 33171886] (Western Blot, Human)
• S An, I Raju, B Surenkhuu, JE Kwon, S Gulati, M Karaman, A Pradeep, S Sinha, C Mun, S Jain Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies Ocul Surf, 2019;0(0):. 2019-01-01 [PMID: 30965123] (Neutralization, Human)
• Bi , Fangfang, Huang , Cao, Tong , Jianbin, Qiu , Guang, Huang , Bo, Wu , Qinxue, Li , Fang, Xu , Zuoshang, Bowser , Robert, Xia , Xu-Gang, Zhou , Hongxia Reactive astrocytes secrete lcn2 to promote neuron death. Proc Natl Acad Sci U S A, 2013;110(10):4069-74. 2013-01-01 [PMID: 23431168] (IHC, Rat)
• Mannelqvist M, Stefansson I, Wik E, Kusonmano K, Raeder M, Oyan A, Kalland K, Moses M, Salvesen H, Akslen L Lipocalin 2 expression is associated with aggressive features of endometrial cancer. BMC Cancer, 2012;12(0):169. 2012-01-01 [PMID: 22559235] (IHC-P, Human)
• Mir SU, Jin L, Craven RJ Neutrophil gelatinase-associated lipocalin (NGAL) expression is dependent on the tumor-associated sigma-2 receptor S2RPgrmc1. J. Biol. Chem., 2012;287(18):14494-501. 2012-01-01 [PMID: 22418433] (Western Blot, Human)
• Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis. J. Immunol., 2008;181(10):7420-7. 2008-01-01 [PMID: 18981165] (IHC-Fr, Human)
• Smith ER, Zurakowski D, Saad A, Scott RM, Moses MA Urinary biomarkers predict brain tumor presence and response to therapy. Clin. Cancer Res., 2008;14(8):2378-86. 2008-01-01 [PMID: 18413828] (IHC-P, Human)
• Gupta K, Shukla M, Cowland JB, Malemud CJ, Haqqi TM Neutrophil gelatinase-associated lipocalin is expressed in osteoarthritis and forms a complex with matrix metalloproteinase 9. Arthritis Rheum., 2007;56(10):3326-35. 2007-01-01 [PMID: 17907186] (Western Blot, Human)
• Pitashny M, Schwartz N, Qing X, Hojaili B, Aranow C, Mackay M, Putterman C Urinary lipocalin-2 is associated with renal disease activity in human lupus nephritis. Arthritis Rheum., 2007;56(6):1894-903. 2007-01-01 [PMID: 17530720] (Western Blot, Human)

Bioinformatics

• Gene Symbol: LCN2
• Uniprot:
  • P80188()