LIN-28B Antibody

Images

 
Western Blot: LIN-28B Antibody [NBP1-50529] - Analysis of anti-LIN28B antibody with HL60 cell line lysates (35ug/lane).
Immunocytochemistry/ Immunofluorescence: LIN-28B Antibody [NBP1-50529] - Fluorescent confocal image of SY5Y cells stained with LIN28B antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 ...read more
Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-50529] - Human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Flow Cytometry: LIN-28B Antibody [NBP1-50529] - Flow cytometric of HL-60 cells using LIN28B Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary ...read more
Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-50529] - Analysis of anti-LIN28B antibody with human spleen at concentration 10 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

LIN-28B Antibody Summary

Immunogen
KLH-conjugated synthetic peptide between amino acids 1-30 from N-terminal region of human LIN28B.
Specificity
Human LIN28B
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIN28B
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:50-1:100
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunofluorescence 1:100

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Purity
Ammonium sulfate precipitation

Alternate Names for LIN-28B Antibody

  • CSDD2
  • CSDD2protein lin-28 homolog B
  • FLJ16517
  • lin-28 homolog B (C. elegans)
  • Lin-28.2
  • LIN28B
  • LIN-28B

Background

LIN28B acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol LIN28B
Entrez
Uniprot