| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32352-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85438-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7726-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21675-50ul | |||
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Analysis in human placenta and prostate tissues. Corresponding LIN-28B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Analysis in human placenta and prostate tissues. Corresponding LIN-28B RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Staining of human kidney, liver, placenta and prostate using Anti-LIN-28B antibody NBP2-32352 (A) shows similar protein distribution across tissues to independent antibody NBP1-85438 (B).](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: LIN-28B Antibody [NBP2-32352] - Analysis in human cell line HEK 293 and human cell line MCF-7.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Western-Blot-NBP2-32352-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28B Antibody [NBP2-32352] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus, nucleoli & cytosol.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32352-img0004.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0019.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0016.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0017.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP2-32352] - Staining of human placenta shows moderate positivity in endothelial cells.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP2-32352-img0018.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0012.jpg "Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0002.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human prostate shows low expression as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human kidney, liver, placenta and testis using Anti-LIN28B antibody NBP1-85438 (A) shows similar protein distribution across tissues to independent antibody NBP2-32352 (B).](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0005.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human liver.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0006.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human testis.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0007.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human kidney.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Analysis in human placenta and prostate tissues. Corresponding LIN-28B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human kidney, liver, placenta and prostate using Anti-LIN-28B antibody NBP1-85438 (A) shows similar protein distribution across tissues to independent antibody NBP2-32352 (B).](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0010.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-85438] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-85438-img0011.jpg)
![Western Blot: LIN-28B Antibody [NBP1-50529] - Analysis of anti-LIN28B antibody with HL60 cell line lysates (35ug/lane).](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Western-Blot-NBP1-50529-img0001.jpg "Western Blot: LIN-28B Antibody [NBP1-50529] - Analysis of anti-LIN28B antibody with HL60 cell line lysates (35ug/lane).")
![Immunocytochemistry/Immunofluorescence: LIN-28B Antibody [NBP1-50529] - Fluorescent confocal image of SY5Y cells stained with LIN28B antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated LIN28B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the LIN28B immunosignal to the cytoplasm.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-50529-img0008.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-50529] - Human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-50529-img0006.jpg)
![Flow Cytometry: LIN-28B Antibody [NBP1-50529] - Flow cytometric of HL-60 cells using LIN28B Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Flow-Cytometry-NBP1-50529-img0010.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-50529] - Analysis of anti-LIN28B antibody with human spleen at concentration 10 ug/ml.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-50529-img0003.jpg)
![Western Blot: LIN-28B Antibody (7D7F7) [NBP3-16815] - Western blot analysis of extracts of various cell lines, using LIN-28B Rabbit mAb (NBP3-16815) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-7D7F7-Western-Blot-NBP3-16815-img0002.jpg "Western Blot: LIN-28B Antibody (7D7F7) [NBP3-16815] - Western blot analysis of extracts of various cell lines, using LIN-28B Rabbit mAb (NBP3-16815) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: LIN-28B Antibody (7D7F7) [NBP3-16815] - Immunohistochemistry of paraffin-embedded human placenta using LIN-28B Rabbit mAb (NBP3-16815) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-7D7F7-Immunohistochemistry-Paraffin-NBP3-16815-img0001.jpg)
![Immunohistochemistry: LIN-28B Antibody (7D7F7) [NBP3-16815] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using LIN-28B Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16815_rabbit-lin-28b-mab-7d7f7-5220251242577.jpg)
![Immunohistochemistry: LIN-28B Antibody (7D7F7) [NBP3-16815] - Immunohistochemistry analysis of paraffin-embedded Human seminoma tissue using LIN-28B Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16815_rabbit-lin-28b-mab-7d7f7-8220252010493.jpg)
![Western Blot: LIN-28B Antibody [NBP3-13029] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with LIN-28B antibody (NBP3-13029) diluted at 1:1000.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Western-Blot-NBP3-13029-img0001.jpg "Western Blot: LIN-28B Antibody [NBP3-13029] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with LIN-28B antibody (NBP3-13029) diluted at 1:1000.")
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP3-13029] - LIN-28B antibody detects LIN-28B protein at cytoplasm and nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. LIN-28B antibody (NBP3-13029) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP3-13029-img0003.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP3-13029] - LIN-28B antibody detects LIN-28B protein at cytoplasm and nucleus in human colon by immunohistochemical analysis. Sample: Paraffin-embedded human colon. LIN-28B antibody (NBP3-13029) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP3-13029-img0002.jpg)
![Western Blot: LIN-28B Antibody (SR1509) [NBP3-21675] - Western blot analysis using NBP3-21675 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21675_rabbit-lin-28b-mab-sr1509-5720231512346.jpg "Western Blot: LIN-28B Antibody (SR1509) [NBP3-21675] - Western blot analysis using NBP3-21675 in K562 cell lysate.")
![Western Blot: Lin28B Overexpression Lysate (Adult Normal) [NBP2-08934] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lin28B.](http://images.novusbio.com/fullsize/Lin28B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08934-img0002.jpg "Western Blot: Lin28B Overexpression Lysate (Adult Normal) [NBP2-08934] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lin28B.")
![SDS-Page: Lin28B Protein [NBP2-23196]](http://images.novusbio.com/fullsize/Lin28B-Protein-SDS-Page-NBP2-23196-img0001.jpg "SDS-Page: Lin28B Protein [NBP2-23196]")