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LIME Antibody

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Western Blot: LIME Antibody [NB100-1436] - (1ug/ml) of Mouse Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot: LIME Antibody [NB100-1436] - (1ug/ml) of Mouse Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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LIME Antibody Summary

Immunogen
Peptide with sequence C-ENVYESIKEMGL corresponding to C-Terminus according to Uniprot Mouse NP_076173.1.
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LIME1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.5 - 2 ug/ml
Application Notes
WB: Approx. 32 kDa band observed in mouse heart lysates (predicted MW of 31 kDa band according to NP_076173.1).
Publications
Read Publication using NB100-1436.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LIME Antibody

  • dJ583P15.4
  • FLJ20406
  • Lck interacting transmembrane adaptor 1
  • Lck-interacting membrane protein
  • Lck-interacting molecule
  • lck-interacting transmembrane adapter 1
  • LIMELP8067

Background

LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LIME Antibody (NB100-1436)(1)


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Bioinformatics

Gene Symbol LIME1
Entrez
Uniprot