MANF Antibody - BSA Free

Images

 
Western Blot: MANF Antibody [NBP1-76836] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MANF recombinant protein per lane. Antibodies: MANF NBP1-76836 (Lane 1: 0.125 ug /mL, Lane 2: 0.25 ...read more
Immunocytochemistry/ Immunofluorescence: MANF Antibody [NBP1-76836] - Immunofluorescence of MANF in Human Brain cells with MANF antibody at 20 ug/mL.
Immunohistochemistry: MANF Antibody [NBP1-76836] - Analysis of MANF in human brain tissue with MANF antibody at 2.5 ug/ml.
Genetic Strategies: Western Blot: MANF Antibody [NBP1-76836] - KO Validation in HEK293T Cells. Loading: 10 ug of HEK293T WT cell lysates or MANF KO cell lysates. Antibodies: MANF NBP1-76836 (1 ug/mL) and ...read more
Western Blot: MANF Antibody [NBP1-76836] - Western Blot Validation in Human, Mouse (NIH/3T3) and Rat (YB2/0) Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: MANF NBP1-76836 (2 ug /mL), 1h incubation at RT in ...read more
Immunohistochemistry-Paraffin: MANF Antibody [NBP1-76836] - Human brain tissue with ARMET antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

MANF Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 12 amino acid synthetic peptide from near the carboxy terminus of human MANF. The immunogen is located within the last 50 amino acids of MANF. Amino Acid Squence: KYAPKAASARTD
Specificity
This antibody does not cross-react with CDNF.
Predicted Species
Bovine (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MANF
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
xxxARMETxxx Protein (NBP1-76836PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for MANF Antibody - BSA Free

  • Arginine-Rich Protein
  • arginine-rich, mutated in early stage tumors
  • ARMET
  • ARP
  • MANF
  • mesencephalic astrocyte-derived neurotrophic factor
  • Protein ARMET

Background

MANF, also known as ARMET, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, MANF enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, MANF has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson's disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress. This antibody does not cross-react with CDNF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MANF
Uniprot