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![Western Blot: LIME Antibody (5D3) [NBP2-45653] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIME1.](http://images.novusbio.com/fullsize/LIME-Antibody-5D3-Western-Blot-NBP2-45653-img0005.jpg "Western Blot: LIME Antibody (5D3) [NBP2-45653] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIME1.")
![Immunohistochemistry: LIME Antibody (5D3) [NBP2-45653] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LIME-Antibody-5D3-Immunohistochemistry-NBP2-45653-img0004.jpg)
![Immunohistochemistry: LIME Antibody (5D3) [NBP2-45653] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LIME-Antibody-5D3-Immunohistochemistry-NBP2-45653-img0001.jpg)
![Immunohistochemistry: LIME Antibody (5D3) [NBP2-45653] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LIME-Antibody-5D3-Immunohistochemistry-NBP2-45653-img0002.jpg)
![Immunohistochemistry: LIME Antibody (5D3) [NBP2-45653] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LIME-Antibody-5D3-Immunohistochemistry-NBP2-45653-img0003.jpg)
![Flow Cytometry: LIME Antibody (LIME-10) - BSA Free [NB500-343] - Anti-Hu LIME1 purified antibody (clone LIME-10) (concentration in sample 1 μg/ml, red-filled histogram) binds specifically to intacellular LIME1 in transfected cells (upper panel), but not to Jurkat cells (lower panel).Level of non-specific binding was assessed using Mouse IgG1 isotype control purified antibody (MOPC-21) under same conditions (concentration in sample 1 μg/ml, black-dashed histogram).](http://images.novusbio.com/fullsize/antibody/nb500-343_mouse-monoclonal-lime-antibody-lime-10-flow-cytometry-1582024145221..jpg)
![Western Blot: LIME Antibody [NB100-1436] - (1ug/ml) of Mouse Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/LIME-Antibody-Western-Blot-NB100-1436-img0002.jpg "Western Blot: LIME Antibody [NB100-1436] - (1ug/ml) of Mouse Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Western Blot: LIME Overexpression Lysate (Adult Normal) [NBL1-12535] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LIME.](http://images.novusbio.com/fullsize/LIME-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12535-img0002.jpg "Western Blot: LIME Overexpression Lysate (Adult Normal) [NBL1-12535] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LIME.")
![Western Blot: LIME Antibody (mLIME-05) [NBP1-47230] - Analysis of mouse thymocyte lysate (1% SDS); non-reduced sample, immunostained by mAb mLIME-05 and goat anti-mouse IgG (H+L)-HRP conjugate.](http://images.novusbio.com/fullsize/LIME-Antibody-mLIME-05-Western-Blot-NBP1-47230-img0001.jpg "Western Blot: LIME Antibody (mLIME-05) [NBP1-47230] - Analysis of mouse thymocyte lysate (1% SDS); non-reduced sample, immunostained by mAb mLIME-05 and goat anti-mouse IgG (H+L)-HRP conjugate.")
![Immunoprecipitation: LIME Antibody (LIME-06) [NBP1-44945] - Induction of LIME tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92; cat. no. NB500-521) or anti-human CD4 (MEM-16), and LIME was immunoprecipitated from laurylmaltoside lysates with the LIME-06 antibody (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom).](http://images.novusbio.com/fullsize/LIME-Antibody-LIME-06-Immunoprecipitation-NBP1-44945-img0003.jpg "Immunoprecipitation: LIME Antibody (LIME-06) [NBP1-44945] - Induction of LIME tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92; cat. no. NB500-521) or anti-human CD4 (MEM-16), and LIME was immunoprecipitated from laurylmaltoside lysates with the LIME-06 antibody (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom).")