LIME Antibody (mLIME-05)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IP
Clone
mLIME-05
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

LIME Antibody (mLIME-05) Summary

Immunogen
Recombinant protein corresponding to amino acids 86-202 of mouse LIME
Specificity
Mouse monoclonal antibody mLIME-05 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
LIME1
Purity
Protein A purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 5 ug/ml
  • Immunoprecipitation 1:50
Application Notes
Western Blot: reducing conditions

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for LIME Antibody (mLIME-05)

  • dJ583P15.4
  • FLJ20406
  • Lck interacting transmembrane adaptor 1
  • Lck-interacting membrane protein
  • Lck-interacting molecule
  • lck-interacting transmembrane adapter 1
  • LIMELP8067

Background

LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for LIME Antibody (NBP1-47230) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LIME1