LIME Antibody (mLIME-05) - BSA Free

Western Blot: LIME Antibody (mLIME-05) [NBP1-47230] - Analysis of mouse thymocyte lysate (1% SDS); non-reduced sample, immunostained by mAb mLIME-05 and goat anti-mouse IgG (H+L)-HRP conjugate.

• Reactivity: Mu
• Applications: WB, IP
• Clone: mLIME-05
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

LIME Antibody (mLIME-05) - BSA Free Summary

• Description: Novus Biologicals Mouse LIME Antibody (mLIME-05) - BSA Free (NBP1-47230) is a monoclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein corresponding to amino acids 86-202 of mouse LIME
• Specificity: Mouse monoclonal antibody mLIME-05 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LIME1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:50
  • Western Blot 1-5 ug/ml
• Application Notes: Western Blot: reducing conditions
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for LIME Antibody (mLIME-05) - BSA Free

• dJ583P15.4
• FLJ20406
• Lck interacting transmembrane adaptor 1
• Lck-interacting membrane protein
• Lck-interacting molecule
• lck-interacting transmembrane adapter 1
• LIMELP8067

Background

LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LIME1