LIME Antibody (LIME-10) - BSA Free

Analysis of human LIME using mouse monoclonal antibody LIME-10 on lysates of J77-LIME transfectants and Jurkat cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 1 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. LIME was detected around 30 kDa.

Flow Cytometry: LIME Antibody (LIME-10) - BSA Free [NB500-343] - Anti-Hu LIME1 purified antibody (clone LIME-10) (concentration in sample 1 μg/ml, red-filled histogram) binds specifically to intacellular LIME1 in transfected cells (upper panel), but not to Jurkat cells (lower panel).Level of non-specific binding was assessed using Mouse IgG1 isotype control purified antibody (MOPC-21) under same conditions (concentration in sample 1 μg/ml, black-dashed histogram).

• Reactivity: Hu
• Applications: WB, Flow, IHC
• Clone: LIME-10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

LIME Antibody (LIME-10) - BSA Free Summary

• Description: Novus Biologicals Mouse LIME Antibody (LIME-10) - BSA Free (NB500-343) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin.
• Specificity: The antibody LIME-10 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LIME1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-5 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot Reducing conditions; 1ug/ml
• Application Notes: Western Blot: Predicted molecular weight 32.3 kDa.
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for LIME Antibody (LIME-10) - BSA Free

• dJ583P15.4
• FLJ20406
• Lck interacting transmembrane adaptor 1
• Lck-interacting membrane protein
• Lck-interacting molecule
• lck-interacting transmembrane adapter 1
• LIMELP8067

Background

LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LIME1
• Entrez:
  • 54923(Human)