LIM Kinase 1/2 [p Thr508] Antibody

Images

 
Western Blot: LIM Kinase 1/2 [p Thr508] Antibody [NBP2-54645] - [p Thr505, p Thr508] Antibody [NBP2-54645] - Western blot analysis of lysed extracts from UV-treated C2C12, PMA-treated HeLa and anisomycin-treated 293 ...read more
Immunohistochemistry-Paraffin: LIM Kinase 1/2 [p Thr505, p Thr508] Antibody [NBP2-54645] - Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK1 (Phospho-Thr508).Bottom ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

LIM Kinase 1/2 [p Thr508] Antibody Summary

Immunogen
A peptide sequence around phosphorylation site of threonine 508 (R-Y-T (p) -V-V) derived from Human LIMK1
Modification
p Thr508
Specificity
This antibody detects endogenous level of LIM Kinase 1/2 only when phosphorylated at threonine 508.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIMK1
Purity
Epitope affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:1000
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Epitope affinity purified

Alternate Names for LIM Kinase 1/2 [p Thr508] Antibody

  • EC 2.7.11.1
  • LIM domain kinase 1
  • LIM motif-containing protein kinase
  • LIMKLIMK-1

Background

Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol LIMK1