PAK1/2/3 [p Thr423, p Thr402, p Thr421] Antibody - BSA Free

Immunohistochemistry-Paraffin: PAK1/2/3 [p Thr423, p Thr402, p Thr421] Antibody [NB100-82131] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-82131(left) or the same antibody preincubated with blocking peptide(right).

Immunocytochemistry/Immunofluorescence: PAK1/2/3 [p Thr423, p Thr402, p Thr421] Antibody [NB100-82131] - Staining of methanol-fixed Hela cells using NB100-82131

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PAK1/2/3 [p Thr423, p Thr402, p Thr421] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PAK1/2/3 [p Thr423, p Thr402, p Thr421] Antibody - BSA Free (NB100-82131) is a polyclonal antibody validated for use in IHC and WB. Anti-PAK1/2/3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human PAK1/2/3 around the phosphorylation site of threonine 423/402/421 (R-S-TP-M-V).
• Modification: p Thr423, p Thr402, p Thr421
• Specificity: Detects endogenous levels of PAK1/2/3 only when phosphorylated at threonine 423/402/421.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Use in Western blot reported in scientific literature (PMID 26821597). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 29066727).
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PAK1/2/3 [p Thr423, p Thr402, p Thr421] Antibody - BSA Free

• alpha-PAK
• EC 2.7.11
• EC 2.7.11.1
• MGC130000
• MGC130001
• p21 protein (Cdc42/Rac)-activated kinase 1
• p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
• p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
• p21-activated kinase 1
• p65-PAK
• PAK-1
• PAKalpha
• serine/threonine-protein kinase PAK 1
• STE20 homolog, yeast

Background

In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAK1/2/3 Antibody (NB100-82131)(2)

Publications using NB100-82131

• Das A, Dines M, Alapin JM, Lamprecht R. Affecting long-term fear memory formation through optical control of Rac1 GTPase and PAK activity in lateral amygdala. Sci Rep. 2017-10-24 [PMID: 29066727] (IHC-Fr, Mouse)
• Jackson WD, Weinrich TW, Woollard KJ. Very-low and low-density lipoproteins induce neutral lipid accumulation and impair migration in monocyte subsets. Sci Rep. 2016-01-29 [PMID: 26821597] (WB, Mouse)

Bioinformatics

• Gene Symbol: PAK1
• Entrez:
  • 5058(Human)
  • 18479(Mouse)
  • 29431(Rat)
• Uniprot:
  • Q13153//Q13177//O75914(Human)