ROCK1 Antibody (154C1465) - (active/cleaved)

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Biological Strategies: Western Blot: cleaved ROCK1 Antibody (154C1465) [NB100-56596] - Analysis using Azide Free version of NB100-56596. Cleaved ROCK-1 in A) untreated, B) 1 hour and C) 4 hour ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, RbSpecies Glossary
Applications WB, ICC/IF
Clone
154C1465
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

ROCK1 Antibody (154C1465) - (active/cleaved) Summary

Immunogen
This antibody was generated by immunizing mice with a synthetic peptide sequence corresponding to amino acids 1114-1126 GNLPESRIEGWLS of human ROCK-1. This sequence is 100% conserved between human, chimpanzee, mouse, rat, dog, and rabbit. This immunogen peptide sequence is adjacent to the caspase-3 cleavage site of ROCK-1.
Specificity
This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for detection of apoptosis.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
ROCK1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 21059388)
Reviewed Applications
Read 1 Review rated 5
using
NB100-56596 in the following applications:

Publications
Read Publications using NB100-56596.

Reactivity Notes

Cross reacts with Chimpanzee.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for ROCK1 Antibody (154C1465) - (active/cleaved)

  • EC 2.7.11
  • EC 2.7.11.1
  • MGC131603
  • MGC43611
  • p160 ROCK-1
  • p160ROCK
  • p160-ROCK
  • PRO0435
  • Renal carcinoma antigen NY-REN-35
  • Rho kinase
  • rho-associated protein kinase 1
  • Rho-associated, coiled-coil containing protein kinase 1
  • Rho-associated, coiled-coil-containing protein kinase 1
  • ROCK1
  • ROK beta

Background

The Rho target protein, Rho-associated coiled coil-containing protein kinase (ROCK)-1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for cleaved ROCK1 Antibody (NB100-56596)(4)

Review for cleaved ROCK1 Antibody (NB100-56596) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56596:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot ROCK1 NB100-56596
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reviewed by:
Anonymous
WB Human 08/01/2018
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Summary

ApplicationWestern Blot
Sample TestedAdult brain
SpeciesHuman
Lot121801A03

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for cleaved ROCK1 Antibody (NB100-56596) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-56596AF350
Alexa Fluor 405 NB100-56596AF405
Alexa Fluor 488 NB100-56596AF488
Alexa Fluor 532 NB100-56596AF532
Alexa Fluor 594 NB100-56596AF594
Alexa Fluor 647 NB100-56596AF647
Alexa Fluor 700 NB100-56596AF700
Alexa Fluor 750 NB100-56596AF750
Biotin NB100-56596B
DyLight 350 NB100-56596UV
DyLight 405 NB100-56596V
DyLight 488 NB100-56596G
DyLight 550 NB100-56596R
DyLight 594 NB100-56596DL594
DyLight 650 NB100-56596C
DyLight 680 NB100-56596FR
DyLight 755 NB100-56596IR
FITC NB100-56596F
HRP NB100-56596H
Janelia Fluor 549 NBP2-33238JF549
Janelia Fluor 646 NBP2-33238JF646

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
08/01/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol ROCK1
Entrez
Uniprot
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