LIF Antibody (39N7D10) - BSA Free

Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - LIF expression in human primary T-cells and cancer cell lines: Jurkat and K562. Image from verified customer review.

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).

Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and (C) human spleen lysate. 3 ug/mL concentration of primary antibody, Goat anti-rat IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this assay.

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of normal human prostate using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Cell surface/membrane- cytoplasmic immunepositivity of LIF was observed specifically in the epithelial cells of prostate alveolar glands, whereas the surrounding fibromuscular stroma cells did not develop any staining.

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunepositivity of LIF was observed in the cuboidal epithelial cells of renal tubules.

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet cells in the rectal glands depicted membrane-cytoplasmic immunostaining of LIF protein.

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed intense membrane-cytoplasmic LIF immunostaining. Additionally, some cells in the lamina propria and the sub-mucosal layer also depicted weak positivity for LIF staining.

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immune positivity of LIF was observed in membranes as well as the cytoplasm of cuboidal epithelial cells of renal tubules.

LIF (39N7D10) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Rat anti-LIF (39N7D10) Protein G-purified Recombinant Monoclonal Antibody (Catalog # NBP2-27406) at 1.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rat IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 40X objective.

LIF (39N7D10) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Rat anti-LIF (39N7D10) Protein G-purified Recombinant Monoclonal Antibody (Catalog # NBP2-27406) at 1.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rat IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clone: 39N7D10
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

LIF Antibody (39N7D10) - BSA Free Summary

• Immunogen: A recombinant murine Lif protein containing amino acids 24-203 was used as the immunogen for this antibody.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: LIF
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 3-5ug/ml~
• Application Notes: The LIF protein can be highly glycosylated and has been observed between 22-34 in Western Blotting. Staining in IHC-P is enhanced through antigen retrieval using 10 mM Sodium Citrate buffer, pH 6.0.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for LIF Antibody (39N7D10) - BSA Free

• CDF
• D Factor
• DIA
• differentiation inhibitory activity
• differentiation stimulating factor
• Differentiation-stimulating factor
• Emfilermin
• HILDA
• HILDAcholinergic differentiation factor
• leukemia inhibitory factor (cholinergic differentiation factor)
• leukemia inhibitory factor
• LIF
• Melanoma-derived LPL inhibitor
• MLPLI

Background

Lif has long been recognized as a cytokine which promotes murine germ cell growth in the absence of feeder cell layers in mouse cells. Characterized initially as an inhibitor of differentiation in M1 murine monocytic leukemia lines (hence the name Leukemia Inhibitory Factor), Lif is now recognized as a key factor for maintaining stem cells in the undifferentiated state- at least in mouse embryonic stem cells. Acting through the Lif receptor and the induced activation of STAT3, murine ESCs can be maintained in vitro in undifferentiated stateHowever, human ESCs, although functionally stimulated with Lif, are not similarly maintained as undifferentiated. This illustrates a different functional program for Lif in mice and humans in maintenance of stem cells. An IL-6 family member, Lif is an important pleiotropic cytokine with activity on cells of hematopoietic and non-hematopoietic origin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIF Antibody (NBP2-27406)(4)

Publications using NBP2-27406

• Kakae, M;Nakajima, H;Tobori, S;Kawashita, A;Miyanohara, J;Morishima, M;Nagayasu, K;Nakagawa, T;Shigetomi, E;Koizumi, S;Mori, Y;Kaneko, S;Shirakawa, H; The astrocytic TRPA1 channel mediates an intrinsic protective response to vascular cognitive impairment via LIF production Science advances 2023-07-21 [PMID: 37478173] (Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Human)
• Yue X, Wang J, Chang CY et al. Leukemia inhibitory factor drives glucose metabolic reprogramming to promote breast tumorigenesis Cell death & disease [PMID: 35440095] (IF/IHC, Human)
• Wang H, Si S, Jiang M Et Al. Leukemia inhibitory factor is involved in the pathogenesis of NSCLC through activation of the STAT3 signaling pathway Oncology Letters 2021-07-14 [PMID: 34386085] (WB, IHC-P)
• Wang H, Wang J, Zhao Y et al. LIF is essential for ISC function and protects against radiation-induced gastrointestinal syndrome Cell Death Dis 2020-07-27 [PMID: 32719388] (IHC-P, ICC/IF, Human)

Review for LIF Antibody (NBP2-27406) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 06/02/2019

Summary

• Application: Western Blot
• Sample Tested: Human T cells
• Species: Human
• Lot: 02094407-04

Comments

• Comments: Samples prepapred in SDS-PAge sampel buffer at 65*C for 10 minutes.

Bioinformatics

• Gene Symbol: LIF
• Uniprot:
  • P09056()