![Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - LIF expression in human primary T-cells and cancer cell lines: Jurkat and K562. Image from verified customer review.](http://images.novusbio.com/fullsize2/LIF-Antibody-(39N7D10)-Western-Blot-NBP2-27406-img0009.jpg "Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - LIF expression in human primary T-cells and cancer cell lines: Jurkat and K562. Image from verified customer review.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IHC-P |
| Clone | 39N7D10 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | A recombinant murine Lif protein containing amino acids 24-203 was used as the immunogen for this antibody. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | LIF |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | The Lif protein can be highly glycosylated and has been observed between 22 kD and 34 kD in western blotting. In IHC-P application, citrate buffer pH 6.0 is not recommended for this antibody. |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
![IL-6 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-6 [Unconjugated]")
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Anonymous |
WB | Human | 06/02/2019 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-27406AF350 | |
| Alexa Fluor 405 | NBP2-27406AF405 | |
| Alexa Fluor 488 | NBP2-27406AF488 | |
| Alexa Fluor 532 | NBP2-27406AF532 | |
| Alexa Fluor 594 | NBP2-27406AF594 | |
| Alexa Fluor 647 | NBP2-27406AF647 | |
| Alexa Fluor 700 | NBP2-27406AF700 | |
| Alexa Fluor 750 | NBP2-27406AF750 | |
| Biotin | NBP2-27406B | |
| DyLight 350 | NBP2-27406UV | |
| DyLight 405 | NBP2-27406V | |
| DyLight 488 | NBP2-27406G | |
| DyLight 550 | NBP2-27406R | |
| DyLight 594 | NBP2-27406DL594 | |
| DyLight 650 | NBP2-27406C | |
| DyLight 680 | NBP2-27406FR | |
| DyLight 755 | NBP2-27406IR | |
| FITC | NBP2-27406F | |
| HRP | NBP2-27406H |
Diseases for LIF Antibody (NBP2-27406)Discover more about diseases related to LIF Antibody (NBP2-27406).
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![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).](http://images.novusbio.com/fullsize2/LIF-Antibody-(39N7D10)-Immunohistochemistry-Paraffin-NBP2-27406-img0008.jpg "Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).")
![Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and (C) human spleen lysate. 3 ug/mL concentration of primary antibody, Goat anti-rat IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this assay.](http://images.novusbio.com/fullsize2/LIF-Antibody-(39N7D10)-Western-Blot-NBP2-27406-img0001.jpg "Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and (C) human spleen lysate. 3 ug/mL concentration of primary antibody, Goat anti-rat IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this assay.")
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of normal human prostate using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Cell surface/membrane- cytoplasmic immunepositivity of LIF was observed specifically in the epithelial cells of prostate alveolar glands, whereas the surrounding fibromuscular stroma cells did not develop any staining.](http://images.novusbio.com/fullsize2/LIF-Antibody-(39N7D10)-Immunohistochemistry-Paraffin-NBP2-27406-img0002.jpg "Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of normal human prostate using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Cell surface/membrane- cytoplasmic immunepositivity of LIF was observed specifically in the epithelial cells of prostate alveolar glands, whereas the surrounding fibromuscular stroma cells did not develop any staining.")
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunepositivity of LIF was observed in the cuboidal epithelial cells of renal tubules.](http://images.novusbio.com/fullsize2/LIF-Antibody-(39N7D10)-Immunohistochemistry-Paraffin-NBP2-27406-img0003.jpg "Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunepositivity of LIF was observed in the cuboidal epithelial cells of renal tubules.")
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet cells in the rectal glands depicted membrane-cytoplasmic immunostaining of LIF protein.](http://images.novusbio.com/fullsize2/LIF-Antibody-(39N7D10)-Immunohistochemistry-Paraffin-NBP2-27406-img0004.jpg "Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet cells in the rectal glands depicted membrane-cytoplasmic immunostaining of LIF protein.")
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed intense membrane-cytoplasmic LIF immunostaining. Additionally, some cells in the lamina propria and the sub-mucosal layer also depicted weak positivity for LIF staining.](http://images.novusbio.com/fullsize2/LIF-Antibody-(39N7D10)-Immunohistochemistry-Paraffin-NBP2-27406-img0006.jpg "Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed intense membrane-cytoplasmic LIF immunostaining. Additionally, some cells in the lamina propria and the sub-mucosal layer also depicted weak positivity for LIF staining.")
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immune positivity of LIF was observed in membranes as well as the cytoplasm of cuboidal epithelial cells of renal tubules.](http://images.novusbio.com/fullsize2/LIF-Antibody-(39N7D10)-Immunohistochemistry-Paraffin-NBP2-27406-img0007.jpg "Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immune positivity of LIF was observed in membranes as well as the cytoplasm of cuboidal epithelial cells of renal tubules.")
![Western Blot: STAT3 Antibody (9D8) [NBP2-22471] - Human breast cancer cell MDA-MB-231 was treated with carboplatin for 72 hours and the expression of p-Stat3 at Y705 and total Stat3 were detected by western blot. From verified customer review.](http://images.novusbio.com/fullsize2/STAT3-Antibody-(9D8)-Western-Blot-NBP2-22471-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of STAT3 using anti-STAT3 (9D8) monoclonal antibody (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing STAT3, at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize2/STAT3-Antibody-(9D8)-Immunocytochemistry-Immunofluorescence-NBP2-22471-img0016.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (9D8) [NBP2-22471] - Biopsies of normal and cancer tissues.](http://images.novusbio.com/fullsize2/STAT3-Antibody-(9D8)-Immunohistochemistry-Paraffin-NBP2-22471-img0017.jpg)
![Western Blot: LRPPRC Antibody [NBP2-38300] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LRPPRC antibody. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize2/LRPPRC-Antibody-Western-Blot-NBP2-38300-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: LRPPRC Antibody [NBP2-38300] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/LRPPRC-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38300-img0004.jpg)
![Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP2-38300] - Staining of human liver.](http://images.novusbio.com/fullsize2/LRPPRC-Antibody-Immunohistochemistry-Paraffin-NBP2-38300-img0011.jpg)
![Western Blot: CCM2 Antibody (1E9) [NBP2-01134] Analysis of extracts (35ug) from 9 different cell lines by using anti-CCM2 monoclonal antibody.](http://images.novusbio.com/fullsize/CCM2 Antibody (1E9)-Western Blot-NBP2-01134-img0007.jpg)
![Immunohistochemistry-Paraffin: CCM2 Antibody (1E9) [NBP2-01134] - Staining of paraffin-embedded Human lymph node tissue using anti-CCM2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CCM2 Antibody (1E9)-Immunohistochemistry-Paraffin-NBP2-01134-img0006.jpg)
![Western Blot: CCM2 Antibody (1E9) [NBP2-01134] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCM2.](http://images.novusbio.com/fullsize/CCM2 Antibody (1E9)-Western Blot-NBP2-01134-img0001.jpg)
![Western Blot: HLA DQA1 Antibody (JU17-34) [NBP2-75525] - Analysis of HLA-DQA1 on different lysates using anti-HLA-DQA1 antibody at 1/1,000 dilution.Positive control:Lane 1: Rat lung Lane 2: Rat skin Lane 3: Mouse thymus Lane 4: Mouse spleenLane 5: Raji](http://images.novusbio.com/fullsize2/HLA DQA1 Antibody (JU17-34)-Western Blot-NBP2-75525-img0004.jpg)
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody (JU17-34) [NBP2-75525] - Analysis of paraffin-embedded rat lung tissue using anti-HLA-DQA1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/HLA-DQA1-Antibody-(JU17-34)-Immunohistochemistry-Paraffin-NBP2-75525-img0003.jpg)
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody (JU17-34) [NBP2-75525] - Analysis of paraffin-embedded human tonsil tissue using anti-HLA-DQA1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/HLA-DQA1-Antibody-(JU17-34)-Immunohistochemistry-Paraffin-NBP2-75525-img0001.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Immunocytochemistry-Immunofluorescence-NB100-524-img0008.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Western Blot: OCT4 Antibody [NB100-2379] - WB detection of OCT4 in mouse brain lysate using OCT4 antibody with ECL based detection. The antibody generated a specific band of OCT4 at the expected ~40kDa position.](http://images.novusbio.com/fullsize2/OCT4-Antibody-Western-Blot-NB100-2379-img0011.jpg)
![Western Blot: OCT4 Antibody [NB100-2379] - The expression of pluripotency factors Oct4 in human head and neck cancer cell lines.](http://images.novusbio.com/fullsize2/OCT4-Antibody-Western-Blot-NB100-2379-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody [NB100-2379] - OCT4 staining in NTERA-2 cells detected with a Dylight 488 labeled secondary antibody.](http://images.novusbio.com/fullsize2/OCT4 Antibody-Immunocytochemistry-Immunofluorescence-NB100-2379-img0008.jpg)
![Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.](http://images.novusbio.com/fullsize2/POMC-Antibody-Western-Blot-NB100-1533-img0005.jpg)
![Immunohistochemistry-Paraffin: POMC Antibody [NB100-1533] - Staining of paraffin embedded Human Pituitary Gland. Antibody at 2 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize2/POMC-Antibody-Immunohistochemistry-Paraffin-NB100-1533-img0003.jpg)
![Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize2/POMC-Antibody-Flow-Cytometry-NB100-1533-img0006.jpg)
![Western Blot: Goat anti-Rat IgG (H+L) Secondary Antibody [HRP] [NB7115] - Detection of DDK in SUSD3 overexpression lysate using anti-DYKDDDDK Epitope Tag antibody (cat.# NBP1-06712) followed by goat anti-rat IgG HRP conjugated secondary antibody. Image from verified customer review.](http://images.novusbio.com/images2/Goat-anti-Rat-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7115-img0003.jpg "Western Blot: Goat anti-Rat IgG (H+L) Secondary Antibody [HRP] [NB7115] - Detection of DDK in SUSD3 overexpression lysate using anti-DYKDDDDK Epitope Tag antibody (cat.# NBP1-06712) followed by goat anti-rat IgG HRP conjugated secondary antibody. Image from verified customer review.")