LIF Antibody (39N7D10)

Images

 
Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - LIF expression in human primary T-cells and cancer cell lines: Jurkat and K562. Image from verified customer review.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).
Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and (C) human spleen lysate. 3 ug/mL concentration of ...read more
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of normal human prostate using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Cell surface/membrane- cytoplasmic ...read more
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunepositivity of ...read more
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet ...read more
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed ...read more
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immune positivity of LIF was observed in ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
39N7D10
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

LIF Antibody (39N7D10) Summary

Immunogen
A recombinant murine Lif protein containing amino acids 24-203 was used as the immunogen for this antibody.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
LIF
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 3 - 5 ug/mL
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
Application Notes
Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32719388)The Lif protein can be highly glycosylated and has been observed between 22 Knockdown Validated and 34 Knockdown Validated in western blotting. In IHC-P application, citrate buffer pH 6.0 is not recommended for this antibody.
Control
LIF Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NBP2-27406 in the following applications:

Publications
Read Publication using
NBP2-27406 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for LIF Antibody (39N7D10)

  • CDF
  • D Factor
  • DIA
  • differentiation inhibitory activity
  • differentiation stimulating factor
  • Differentiation-stimulating factor
  • Emfilermin
  • HILDA
  • HILDAcholinergic differentiation factor
  • leukemia inhibitory factor (cholinergic differentiation factor)
  • leukemia inhibitory factor
  • LIF
  • Melanoma-derived LPL inhibitor
  • MLPLI

Background

Lif has long been recognized as a cytokine which promotes murine germ cell growth in the absence of feeder cell layers in mouse cells (1-3)Characterized initially as an inhibitor of differentiation in M1 murine monocytic leukemia lines (hence the name Leukemia Inhibitory Factor),Lif is now recognized as a key factor for maintaining stem cells in the undifferentiated state- at least in mouse embryonic stem cells (4)Acting through the Lif receptor and the induced activation of STAT3, murine ESCs can be maintained in vitro in undifferentiated stateHowever, human ESCs, although functionally stimulated with Lif, are not similarly maintained as undifferentiatedThis illustrates a different functional program for Lif in mice and humans in maintenance of stem cellsAn IL-6 family member, Lif is an important pleiotropic cytokine with activity on cells of hematopoietic and non-hematopoietic origin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LIF Antibody (NBP2-27406)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IHC-P.


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ICC/IF
(1)
IHC-P
(1)
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Human
(1)
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Review for LIF Antibody (NBP2-27406) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human .

Reviews using NBP2-27406:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot LIF NBP2-27406
Enlarge
4
reviewed by:
Anonymous
WB Human 06/02/2019
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Summary

ApplicationWestern Blot
Sample TestedHuman T cells
SpeciesHuman
Lot02094407-04

Comments

CommentsSamples prepapred in SDS-PAge sampel buffer at 65*C for 10 minutes.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LIF Antibody (NBP2-27406) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-27406AF350
Alexa Fluor 405 NBP2-27406AF405
Alexa Fluor 488 NBP2-27406AF488
Alexa Fluor 532 NBP2-27406AF532
Alexa Fluor 594 NBP2-27406AF594
Alexa Fluor 647 NBP2-27406AF647
Alexa Fluor 700 NBP2-27406AF700
Alexa Fluor 750 NBP2-27406AF750
Biotin NBP2-27406B
DyLight 350 NBP2-27406UV
DyLight 405 NBP2-27406V
DyLight 488 NBP2-27406G
DyLight 550 NBP2-27406R
DyLight 594 NBP2-27406DL594
DyLight 650 NBP2-27406C
DyLight 680 NBP2-27406FR
DyLight 755 NBP2-27406IR
FITC NBP2-27406F
HRP NBP2-27406H

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Bioinformatics Tool for LIF Antibody (NBP2-27406)

Discover related pathways, diseases and genes to LIF Antibody (NBP2-27406). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for LIF Antibody (NBP2-27406)

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Pathways for LIF Antibody (NBP2-27406)

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PTMs for LIF Antibody (NBP2-27406)

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Blogs on LIF.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
06/02/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol LIF
Uniprot