LIF Antibody (139124) [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clone: 139124
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

LIF Antibody (139124) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse LIF
  Pro25-Phe203
• Specificity: Detects mouse LIF in direct ELISAs and Western blots. In Western blots, no cross‑reactivity with recombinant mouse (rm) IL-6, rmIL-11, recombinant human (rh) LIF, rmCT-1, rhCLC or recombinant rat CNTF is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: LIF
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIF Antibody (139124) [Unconjugated]

• CDF
• D Factor
• DIA
• differentiation inhibitory activity
• differentiation stimulating factor
• Differentiation-stimulating factor
• Emfilermin
• HILDA
• HILDAcholinergic differentiation factor
• leukemia inhibitory factor (cholinergic differentiation factor)
• leukemia inhibitory factor
• LIF
• Melanoma-derived LPL inhibitor
• MLPLI

Background

Leukemia inhibitory factor (LIF) was initially identified as a factor that inhibited the proliferation and induced the differentiation to macrophages of the murine myeloid leukemic cell line M1. Subsequent to its purification and molecular cloning, LIF was recognized to be a pleiotropic factor with multiple effects on both hematopoietic and non-hematopoietic cells. LIF has overlapping biological functions with OSM, IL-6, IL-11 and CNTF. All these cytokines utilize gp130 as a component in their signal transducing receptor complexes. Mouse LIF cDNA encodes a 203 amino acid residue polypeptide with a 23 amino acid signal peptide that is cleaved to yield a 180 amino acid mature mouse LIF. Native human and mouse LIF are highly glycosylated monomeric proteins. Both human and murine LIF protein sequences have multiple potential N- and O-linked glycosylation sites and six conserved cysteine residues that are involved in three intramolecular disulfide bridges.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIF Antibody (MAB449)(3)

Publications using MAB449

• KP Kim, Y Wu, J Yoon, K Adachi, G Wu, S Velychko, CM MacCarthy, B Shin, A Röpke, MJ Arauzo-Bra, M Stehling, DW Han, Y Gao, J Kim, S Gao, HR Schöler Reprogramming competence of OCT factors is determined by transactivation domains Science Advances, 2020-09-02;6(36):. 2020-09-02 [PMID: 32917606] (Inhibition, Mouse)
• Moidunny S, Vinet J Adenosine A2B receptor-mediated leukemia inhibitory factor release from astrocytes protects cortical neurons against excitotoxicity. J Neuroinflammation, 2012-08-16;9(1):198. 2012-08-16 [PMID: 22894638] (ICC, Western Blot, Mouse)
• Gard AL, Gavin E, Solodushko V, Pennica D Cardiotrophin-1 in choroid plexus and the cerebrospinal fluid circulatory system. Neuroscience, 2004-01-01;127(1):43-52. 2004-01-01 [PMID: 15219667] (Western Blot, Mouse, Rat)

Bioinformatics

• Gene Symbol: LIF