Lgr5/GPR49 Antibody (803420) [Phycoerythrin]

HEK293 human embryonic kidney cell line transfected with either (A) mouse Lgr5/GPR49 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse Lgr5/GPR49 PE-conjugated Monoclonal Antibody (Catalog # FAB8240P). Quadrant markers were set based on control antibody staining (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 803420
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

Lgr5/GPR49 Antibody (803420) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant mouse Lgr5/GPR49
  Lys375-Pro547
  Accession # Q9Z1P4
• Specificity: Detects mouse Lgr5/GPR49 in ELISAs. Stains mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD20", has been found to detect an epitope in C-terminal LRR cap of Lgr5 (Ref. 1).
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: LGR5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lgr5/GPR49 Antibody (803420) [Phycoerythrin]

• FEX
• G protein-coupled receptor 49
• GPR49
• GPR49G-protein coupled receptor HG38
• GPR67
• G-protein coupled receptor 49
• G-protein coupled receptor 67
• GRP49
• HG38
• leucine-rich repeat containing G protein-coupled receptor 5
• leucine-rich repeat-containing G protein-coupled receptor 5
• leucine-rich repeat-containing G-protein coupled receptor 5
• Lgr5
• MGC117008
• orphan G protein-coupled receptor HG38

Background

Leucine‑rich repeat G‑protein‑coupled receptor 5 (Lgr5), also called GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (2). Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the mouse Lgr5 long extracellular domain used as an immunogen shares 90% and 95% amino acid sequence identity with human and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells (3). Lgr5⁺ stem cells can produce all epithelial cell types of the intestinal crypts (4). It is upregulated in stem cells that give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas (5,6). This antibody has been referred to as "RD20" by Peng et al.(1).

1. Peng et al. (2013) Cell Rep. 3(6):1885.
2. de Lau et al. (2014) Genes Dev. 28(4):305.
3. Barker et al. (2013) Development 140(12):2484.
4. Clevers (2013) Nature 495(7439):53.
5. Wu et al. (2014) Nat Commun 5:3149.
6. Jang et al. (2013) PLoS One 8(12):e82390495.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lgr5/GPR49 Antibody (FAB8240P)(3)

Publications using FAB8240P

• HY Chiang, HH Lu, JN Sudhakar, YW Chen, NS Shih, YT Weng, JW Shui IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence Nature Communications, 2022-02-15;13(1):874. 2022-02-15 [PMID: 35169117] (Flow Cytometry, Mouse)
• B Kumar, AK Adebayo, M Prasad, ML Capitano, R Wang, P Bhat-Naksh, M Anjanappa, E Simpson, D Chen, Y Liu, JM Schilder, AB Colter, C Maguire, CJ Temm, G Sandusky, EH Doud, AB Wijeratne, AL Mosley, HE Broxmeyer, H Nakshatri Tumor collection/processing under physioxia uncovers highly relevant signaling networks and drug sensitivity Science Advances, 2022-01-12;8(2):eabh3375. 1/12/2022 [PMID: 35020422]
• Y Li, M Chen, J Hu, R Sheng, Q Lin, X He, M Guo Volumetric Compression Induces Intracellular Crowding to Control Intestinal Organoid Growth via Wnt/&beta-Catenin Signaling Cell Stem Cell, 2020-10-13;0(0):. 2020-10-13 [PMID: 33053374] (ICC, Immunoprecipitation, Human)

Bioinformatics

• Gene Symbol: LGR5
• Uniprot:
  • Q9Z1P4()