Lgr5/GPR49 Antibody (803420) [mFluor Violet 500 SE]

• Reactivity: Mu
• Applications: Flow, CyTOF-ready, Func, ICC/IF
• Clone: 803420
• Clonality: Monoclonal
• Host: Rat
• Conjugate: mFluor Violet 500 SE

Lgr5/GPR49 Antibody (803420) [mFluor Violet 500 SE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Additional Information: Published as Clone RD20
• Immunogen: E. coli-derived recombinant mouse Lgr5/GPR49
  Lys375-Pro547
  Accession # Q9Z1P4
• Specificity: Detects mouse Lgr5/GPR49 in ELISA. Stains mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD20", has been found to detect an epitope in C-terminal LRR cap of LGR5 (Ref. 1).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: LGR5
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Agonist Activity
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product MAB8240. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Lgr5/GPR49 Antibody (803420) [mFluor Violet 500 SE]

• FEX
• G protein-coupled receptor 49
• GPR49
• GPR49G-protein coupled receptor HG38
• GPR67
• G-protein coupled receptor 49
• G-protein coupled receptor 67
• GRP49
• HG38
• leucine-rich repeat containing G protein-coupled receptor 5
• leucine-rich repeat-containing G protein-coupled receptor 5
• leucine-rich repeat-containing G-protein coupled receptor 5
• Lgr5
• MGC117008
• orphan G protein-coupled receptor HG38

Background

Leucine-rich repeat-containing LGR5, also known as GPR49, is an Orphan-A G-protein coupled receptor with an unknown ligand. GPR49 is a stem cell marker of the intestinal epithelium and the hair follicule, and is often expressed in the brain, skeletal muscle, placenta, and spinal cord. Because LGR5 is typically found in mouse hair follicles it may potentially be used to regrow hair in mice. GPR49/LGR5 antibodies are useful tools for research on hair loss and hair follicule development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LGR5