Lgr5/GPR49 Antibody (707042)

Images

 
Lgr5/GPR49 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with GFP (green) tagged mouse LGR5 using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) at 10 µg/mL ...read more
Lgr5/GPR49 was detected in immersion fixed NSO mouse myeloma cell line transfected with GFP (green) tagged human LGR5 using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) at 10 µg/mL for 3 hours at ...read more
Lgr5/GPR49 was detected in MYC tagged human transfectants using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078). Cells were stained using an anti-mouse HRP-conjugated secondary antibody (brown). ...read more
Lgr5/GPR49 was detected in immersion fixed MYC tagged mouse transfectants using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078). Cells were stained using an anti-mouse HRP-conjugated secondary ...read more
NS0 mouse myeloma cell line transfected with human Lgr5/GPR49 and eGFP was stained with and either (A) Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) or (B) Mouse IgG2A Isotype Control (Catalog # ...read more
Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) induces TOPflash activity in the HEK293 human embryonic kidney cell line stably expressing LGR5 in the presence of Wnt-3a, but in the absence of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready, Func, ICC/IF
Clone
707042
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Lgr5/GPR49 Antibody (707042) Summary

Additional Information
Published as Clone RD42
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Lgr5/GPR49
Met1-Ile560
Accession # O75473
Specificity
Detects human Lgr5/GPR49 in ELISA. Stains human and mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD42", has been found to detect an epitope in C-terminal LRR cap of LGR5 (Ref. 1).
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
LGR5
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • Agonist Activity
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
Control
Lgr5/GPR49 Knockout HeLa Cell Lysate
Publications
Read Publications using
MAB8078 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lgr5/GPR49 Antibody (707042)

  • FEX
  • G protein-coupled receptor 49
  • GPR49
  • GPR49G-protein coupled receptor HG38
  • GPR67
  • G-protein coupled receptor 49
  • G-protein coupled receptor 67
  • GRP49
  • HG38
  • leucine-rich repeat containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G-protein coupled receptor 5
  • Lgr5
  • MGC117008
  • orphan G protein-coupled receptor HG38

Background

Leucine‑rich repeat G‑protein‑coupled receptor 5 (Lgr5), also called GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (2). Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the human Lgr5 long extracellular domain used as an immunogen shares 90% amino acid sequence identity with mouse and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells (3). Lgr5+ stem cells can produce all epithelial cell types of the intestinal crypts (4). Abnormal LGR5 expression and regulation in stem cells might give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas (5,6). This antibody has been referred to as "RD42" in Peng et al. (1).
  1. Peng et al. (2013) Cell Rep. 3(6):1885.
  2. de Lau et al. (2014) Genes Dev. 28(4):305.
  3. Barker et al. (2013) Development 140(12):2484.
  4. Clevers (2013) Nature 495(7439):53.
  5. Wu et al. (2014) Nat Commun 5:3149.
  6. Jang et al. (2013) PLoS One 8(12): e82390495.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB120-16518
Species: Hu, Mu, Rt, Po
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-47386
Species: Hu, Mu, Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, Flow-IC
NBP1-96140
Species: Hu, Mu
Applications: WB, ChIP, Flow, ICC/IF, IP
MAB8458
Species: Hu
Applications: Flow, CyTOF-ready, ICC
AF2628
Species: Hu, Mu, Rt
Applications: WB, IHC, ICC
NBP1-77127
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NLS3593
Species: Hu, Po, Bv, Ca, Eq, Mk, Pm, Rb
Applications: IHC, IHC-P
NB100-2379
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, Flow, ICC/IF, IHC
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
AF1172
Species: Hu, Mu, Rt, Ca
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP2-45041
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
NB100-77903
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-Fr
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Lgr5/GPR49 Antibody (MAB8078)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, Western Blot.


Filter By Application
IHC-P
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(2)
All Species

Reviews for Lgr5/GPR49 Antibody (MAB8078) (0)

There are no reviews for Lgr5/GPR49 Antibody (MAB8078). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Lgr5/GPR49 Antibody (MAB8078) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB8078U
Alexa Fluor 405 FAB8078V
Alexa Fluor 488 FAB8078G
Alexa Fluor 594 FAB8078T
Alexa Fluor 647 FAB8078R
Alexa Fluor 700 FAB8078N
Alexa Fluor 750 FAB8078S
Allophycocyanin FAB8078A
Phycoerythrin FAB8078P

Additional Lgr5/GPR49 Products

Bioinformatics Tool for Lgr5/GPR49 Antibody (MAB8078)

Discover related pathways, diseases and genes to Lgr5/GPR49 Antibody (MAB8078). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Lgr5/GPR49 Antibody (MAB8078)

Discover more about diseases related to Lgr5/GPR49 Antibody (MAB8078).
 

Pathways for Lgr5/GPR49 Antibody (MAB8078)

View related products by pathway.

PTMs for Lgr5/GPR49 Antibody (MAB8078)

Learn more about PTMs related to Lgr5/GPR49 Antibody (MAB8078).

Blogs on Lgr5/GPR49.

Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.

Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Lgr5/GPR49 Antibody (707042) and receive a gift card or discount.

Bioinformatics

Gene Symbol LGR5
Uniprot
Novus 100% Guarantee