NS0 mouse myeloma cell line transfected with human Lgr5/GPR49 and eGFP was stained with either (A) Mouse Anti-Human Lgr5/GPR49 PE‑conjugated Monoclonal Antibody (Catalog # FAB8078P) or (B) Mouse IgG2A Phycoerythrin ...read more
Chinese hamster ovary cell line CHO-derived recombinant human Lgr5/GPR49 Met1-Ile560 Accession # O75473
Detects human Lgr5/GPR49 in ELISAs. Stains human and mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD42", has been found to detect an epitope in the C-terminal LRR cap of Lgr5 (Ref. 1).
Protein A or G purified from hybridoma culture supernatant
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Leucine‑rich repeat G‑protein‑coupled receptor 5 (Lgr5), also called GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (2). Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the human Lgr5 long extracellular domain used as an immunogen shares 90% amino acid sequence identity with mouse and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells (3). Lgr5+ stem cells can produce all epithelial cell types of the intestinal crypts (4). Abnormal Lgr5 expression and regulation in stem cells might give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas (5,6). This antibody has been referred to as "RD42" in Peng et al. (1).
Peng et al. (2013) Cell Rep. 3(6):1885.
de Lau et al. (2014) Genes Dev. 28(4):305.
Barker et al. (2013) Development 140(12):2484.
Clevers (2013) Nature 495(7439):53.
Wu et al. (2014) Nat Commun 5:3149.
Jang et al. (2013) PLoS One 8(12): e82390495.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.