Lgr5/GPR49 Antibody (707042) [Phycoerythrin]

Images

 
NS0 mouse myeloma cell line transfected with human Lgr5/GPR49 and eGFP was stained with either (A) Mouse Anti-Human Lgr5/GPR49 PE‑conjugated Monoclonal Antibody (Catalog # FAB8078P) or (B) Mouse IgG2A Phycoerythrin ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
707042
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Lgr5/GPR49 Antibody (707042) [Phycoerythrin] Summary

Additional Information
Published as Clone RD42
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Lgr5/GPR49
Met1-Ile560
Accession # O75473
Specificity
Detects human Lgr5/GPR49 in ELISAs. Stains human and mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD42", has been found to detect an epitope in the C-terminal LRR cap of Lgr5 (Ref. 1).
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
LGR5
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publication using
FAB8078P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lgr5/GPR49 Antibody (707042) [Phycoerythrin]

  • FEX
  • G protein-coupled receptor 49
  • GPR49
  • GPR49G-protein coupled receptor HG38
  • GPR67
  • G-protein coupled receptor 49
  • G-protein coupled receptor 67
  • GRP49
  • HG38
  • leucine-rich repeat containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G-protein coupled receptor 5
  • Lgr5
  • MGC117008
  • orphan G protein-coupled receptor HG38

Background

Leucine‑rich repeat G‑protein‑coupled receptor 5 (Lgr5), also called GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (2). Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the human Lgr5 long extracellular domain used as an immunogen shares 90% amino acid sequence identity with mouse and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells (3). Lgr5+ stem cells can produce all epithelial cell types of the intestinal crypts (4). Abnormal Lgr5 expression and regulation in stem cells might give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas (5,6). This antibody has been referred to as "RD42" in Peng et al. (1).
  1. Peng et al. (2013) Cell Rep. 3(6):1885.
  2. de Lau et al. (2014) Genes Dev. 28(4):305.
  3. Barker et al. (2013) Development 140(12):2484.
  4. Clevers (2013) Nature 495(7439):53.
  5. Wu et al. (2014) Nat Commun 5:3149.
  6. Jang et al. (2013) PLoS One 8(12): e82390495.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lgr5/GPR49 Antibody (FAB8078P)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB8078U
Alexa Fluor 405 FAB8078V
Alexa Fluor 488 FAB8078G
Alexa Fluor 594 FAB8078T
Alexa Fluor 647 FAB8078R
Alexa Fluor 700 FAB8078N
Alexa Fluor 750 FAB8078S
Allophycocyanin FAB8078A
Phycoerythrin FAB8078P

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Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.

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Bioinformatics

Gene Symbol LGR5
Uniprot
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