Lewis A Blood Group Antigen Antibody (7LE)

Images

 
Immunohistochemistry-Paraffin: Lewis A Blood Group Antigen Antibody (7LE) [NB500-525] - Staining of human pancreas (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE).
Immunohistochemistry-Paraffin: Lewis A Blood Group Antigen Antibody (7LE) [NB500-525] - staining of colorectal adenocarcinoma (paraffin-embedded sections) with anti-FUT3/Blood Group Lewis A (7LE).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
7LE
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Lewis A Blood Group Antigen Antibody (7LE) Summary

Immunogen
Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification).
Specificity
The antibody 7LE recognizes Lewisa blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. Lewisa may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ABO
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
Purity is > 95% (by SDS-PAGE). For Immunohistochemistry-Paraffin use heat mediated antigen retrieval (sodium citrate).
Publications
Read Publications using NB500-525.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Background

This antibody recognizes FUT3/Blood Group Lewis A, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. FUT3/Blood Group Lewis A may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lewis A Blood Group Antigen Antibody (NB500-525)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ABO
Entrez
Uniprot