Lewis A Blood Group Antigen Antibody (7LE) - BSA Free

Immunohistochemistry: Lewis A Blood Group Antigen Antibody (7LE) [NB500-525] - Staining of human colon adenocarcinoma (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE).

Immunohistochemistry-Paraffin: Lewis A Blood Group Antigen Antibody (7LE) [NB500-525] - Staining of colorectal adenocarcinoma with anti-FUT3/Blood Group Lewis A (7LE).

Immunohistochemistry-Paraffin: Lewis A Blood Group Antigen Antibody (7LE) [NB500-525] - Staining of human pancreas (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE).

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 7LE
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Lewis A Blood Group Antigen Antibody (7LE) - BSA Free Summary

• Description: Novus Biologicals Mouse Lewis A Blood Group Antigen Antibody (7LE) - BSA Free (NB500-525) is a monoclonal antibody validated for use in IHC, WB and ELISA. Anti-Lewis A Blood Group Antigen Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification).
• Specificity: The antibody 7LE recognizes Lewisa blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. Lewisa may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABO
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:1000
• Application Notes: Purity is > 95% (by SDS-PAGE). For IHC-P use heat mediated antigen retrieval (sodium citrate).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Lewis A Blood Group Antigen Antibody (7LE) - BSA Free

• A transferase
• A3GALNT
• A3GALT1
• ABO
• B transferase
• EC=2.4.1.37
• EC=2.4.1.40
• Fucosylglycoprotein 3-alpha-galactosyltransferase
• Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form
• Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
• Glycoprotein-fucosylgalactoside alpha-galactosyltransferase
• Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase
• GTB
• Histo-blood group A transferase
• Histo-blood group ABO system transferase
• Histo-blood group B transferase
• NAGAT

Background

This antibody recognizes FUT3/Blood Group Lewis A, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. FUT3/Blood Group Lewis A may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lewis A Blood Group Antigen Antibody (NB500-525)(2)

Publications using NB500-525

• Creuzot-Garcher C, Guerzider V, Assem M et al. Alteration of sialyl Lewis epitope expression in pterygium. Invest Ophthalmol Vis Sci. 1999-07-01 [PMID: 10393028]
• First International Workshop on Monoclonal Antibodies Against Red Blood Cell and Related Antigens. Paris, September 21-24, 1987. Vol. II: Other blood group systems. Rev Fr Transfus Immunohematol. 1988-04-01 [PMID: 3212306]

Bioinformatics

• Gene Symbol: ABO
• Entrez:
  • 2525(Human)
• Uniprot:
  • P21217()